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Improved quantitative method for isolating Calonectria ilicicola from naturally infested soils

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Abstract

We developed an improved procedure for quantitative isolation of Calonectria ilicicola from naturally infested soils. The selective medium contained 20 g l-sorbose, 4 g yeast extract, 12.5 mg flutolanil, 1.5 mg thiabendazole, 40 mg chlortetracycline hydrochloride, 10 mg chloramphenicol, 1 mL tergitol, 20 g agar, and up to 1 L with distilled water. Soil samples are wet-sieved (mesh size 0.250–0.038 mm) and then surface-sterilized for 30 s with 0.25 % NaClO solution before incubation with this medium. Compared with previously reported methods, this method was more effective for isolating C. ilicicola, and the amount of contamination was decreased.

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Acknowledgment

We thank Dr. Tomohisa Kuroda, Niigata Agricultural Research Institute, for providing soil samples.

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Authors and Affiliations

  1. National Agriculture Research Center, 3-1-1 Kannondai, Tsukuba, Ibaraki, 305-8666, Japan

    Sunao Ochi & Akio Nakagawa

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  1. Sunao Ochi
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  2. Akio Nakagawa
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Correspondence to Sunao Ochi.

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Ochi, S., Nakagawa, A. Improved quantitative method for isolating Calonectria ilicicola from naturally infested soils. J Gen Plant Pathol 78, 147–150 (2012). https://doi.org/10.1007/s10327-012-0377-7

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  • DOI: https://doi.org/10.1007/s10327-012-0377-7

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