Abstract
In 2008, bacterial rot on onion bulbs (Allium cepa L.) was observed in several low-temperature warehouses in Changnyeong-gun, Korea. The causal pathogen was isolated and identified as Bacillus amyloliquefaciens based on morphological and biochemical characterization and sequence analysis of its genome. The isolated bacteria caused the same rot symptom on inoculated onion bulbs as found in naturally infected onions during storage and was reisolated from these bulbs. This is the first report of bacterial rot of onion caused by B. amyloliquefaciens in Korea.
References
Chun J, Bae KS (2000) Phylogenetic analysis of Bacillus subtilis and related taxa based on partial gyrA gene sequence. Antonie van Leewenhoek 78:123–127
Ezaki T, Hashimoto Y, Yabuuchi E (1989) Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229
Galal AA, El-Bana AA, Janse J (2006) Bacillus pumilus, a new pathogen on mango plants. Egypt J Phytopathol 34:17–29
Hansen MC, Tolker-Nielsen T, Givskov M, Molin S (1998) Biased 16s rDNA PCR amplification caused by interference from DNA flanking the template region. FEMS Microbiol Ecol 26:141–149
Kim YK, Lee SB, Lee SY, Lee YH, Kim HD, Yun T, Park SW (2001) Ecology and control of postharvest disease of garlics and onions. In: Chung MN (ed) Crop Protection Research (2001). National Institute of Agricultural Science and Technology, Suwon, pp 56–89
Kwon JH, Jee HJ (2007) Occurrence of stem root of Disporum smilacinum caused by Sclerotium rolfsii in Korea. Plant Pathol J 23:212–214
Lee CJ, Lim SK, Kim BC, Park W (2005) Characterization of bacteria isolated from rotted onion (Aliiumcepa L.). Kor J Microbiol Biotechnol 33:248–254
Roberts MS, Nakamura LK, Cohan FM (1994) Bacillus mojavensis sp. nov., distinguishable from Bacillus subtilis by sexual isolation, divergence in DNA sequence, and differences in fatty acid composition. Int J Syst Bacteriol 44:256–264
Ruiz-García C, Béjar V, Martínez-Checa F, Llamas I, Quesada E (2005) Bacillus velezensis sp. nov., a surfactant-producing bacterium isolated from the river Vélez in Málaga, southern Spain. Int J Syst Evol Microbiol 55:191–195
Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
Saleh OI (1995) Identification of phytopathogenic bacteria associated with a post harvest disease of garlic cloves in relation to cell-wall degrading enzymes. Egypt J Microbiol 30:177–202
Saleh OI, Huang PY, Huang JS (1997) Bacillus pumilus, the cause of bacterial blotch of immature balady peach in Egypt. J Phytopathol 145:447–453
Stackebrandt E, Goebel BM (1994) Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849
Wang LT, Lee FL, Tai CJ, Kasai H (2007) Comparison of gyrB gene sequences, 16S rRNA gene sequences and DNA–DNA hybridization in the Bacillus subtilis group. Int J Syst Evol Microbiol 57:1846–1850
Wang LT, Lee FL, Tai CJ, Kuo HP (2008) Bacillus velezensis is a later heterotypic synonym of Bacillus amyloliquefaciens. Int J Syst Evol Microbiol 58:671–675
Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O, Krichevsky MI, Moore LH, Moore WEC, Murray RGE, Stackebrandt E, Starr MP, Trüper HG (1987) Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464
Wright PJ, Clark RG, Hale CN (1993) A storage soft rot of New Zealand onions caused by Pseudomonas gladioli pv. alliicola. N Z J Crop Hort Sci 21:225–227
Yamamoto S, Harayama S (1995) PCR amplification and direct sequencing of gyrBgenes with universal primers and their application to the detection and taxonomic analysis of Pseudomonas putida strains. Appl Environ Microbiol 61:1104–1109
Acknowledgment
This work was carried out with the support of “Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ00692305)” Rural Development Administration, Republic of Korea.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Hwang, SK., Back, CG., Win, N.K.K. et al. Occurrence of bacterial rot of onion caused by Bacillus amyloliquefaciens in Korea. J Gen Plant Pathol 78, 227–232 (2012). https://doi.org/10.1007/s10327-012-0376-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10327-012-0376-8