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Detection and analysis of genetic variations in GOB locus of Plasmopara viticola by DNA sequence analysis

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Abstract

The population genetic structure of Plasmopara viticola in Japan was analyzed using grapevine downy mildew specimens collected from two islands, Honshu and Hokkaido. By simple sequence repeat analysis with the GOB microsatellite marker and DNA sequencing, an accurate copy number of the (CT) n (CTAT) n repeat was determined. Consequently, we found a large number of genetic variations in (CT) n and (CTAT) n repeats. Also, a single nucleotide polymorphism in the (CTAT) n repeat of the GOB locus was detected. These genetic variations may serve as a valuable tool to understand the population structure of P. viticola.

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Authors and Affiliations

  1. The Institute of Enology and Viticulture, University of Yamanashi, Kofu, Yamanashi, 400-0005, Japan

    Mai Mochizuki, Yoshinao Aoki & Shunji Suzuki

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  1. Mai Mochizuki
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  2. Yoshinao Aoki
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  3. Shunji Suzuki
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Correspondence to Shunji Suzuki.

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Mochizuki, M., Aoki, Y. & Suzuki, S. Detection and analysis of genetic variations in GOB locus of Plasmopara viticola by DNA sequence analysis. J Gen Plant Pathol 78, 170–175 (2012). https://doi.org/10.1007/s10327-012-0368-8

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  • DOI: https://doi.org/10.1007/s10327-012-0368-8

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