Novel PCR primers for detection of genetically diverse virulent Agrobacterium tumefaciens biovar 1 strains
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- Yakabe, L.E., Maccree, M.M., Sudarshana, P. et al. J Gen Plant Pathol (2012) 78: 121. doi:10.1007/s10327-012-0364-z
Novel PCR primers were developed to amplify a 243-bp fragment of an intergenic region between gene 5 and tms2 on the T-DNA of Agrobacterium tumefaciens biovar 1. These primers exhibit 100% positive correlation with strain virulence, 100% negative correlation with avirulence, and did not generate extraneous bands, thus facilitating robust real-time PCR detection.