Advertisement

Journal of General Plant Pathology

, Volume 77, Issue 4, pp 253–256 | Cite as

Molecular characterization of Potato spindle tuber viroid in dahlia

  • Taro Tsushima
  • Sakiko Murakami
  • Hirotsugu Ito
  • Ying-Hong He
  • Adkar Purushothama Charith Raj
  • Teruo SanoEmail author
Viral and Viroid Diseases

Abstract

A new variant of Potato spindle tuber viroid (PSTVd) was detected for the first time from dahlia grown in Japan. The dahlia isolate of PSTVd formed a quasi-species and a major sequence variant consisting of 361 nucleotides in length, including five substitutions, three insertions, and one deletion, when compared to the intermediate strain from potato. In bioassays with the new isolate, Rutgers tomato developed mild stunting and leaf curling.

Keywords

Potato spindle tuber viroid Dahlia 

Notes

Acknowledgments

This work was supported in part by Grants-in Aid for Scientific Research B 21380029, for Foreign JSPS Fellow 22-00404 from Japan Society for the Promotion of Science, and Grant-in Aid-for Scientific Research of Priority Area, Hirosaki University 2009 and 2010. This work was done in part at Gene Research Center, Hirosaki University.

References

  1. Hataya T (2009) Duplex reverse transcription-polymerase chain reaction system to detect Potato spindle tuber viroid using an internal control mRNA and a non-infectious positive control RNA. J Gen Plant Pathol 75:167–172CrossRefGoogle Scholar
  2. Kawaguchi-Ito Y, Li S-F, Tagawa M, Araki H, Goshono M, Yamamoto S, Tanaka M, Narita M, Tanaka K, Liu S-X, Shikata E, Sano T (2009) Cultivated grapevines represent a symptomless reservoir for the transmission of Hop stunt viroid to hop crops: 15 years of evolutionary analysis. PLoS ONE 4(12):e8386. doi: 10.1371/journal.pone.0008386 PubMedCrossRefGoogle Scholar
  3. Levy L, Lee I-M, Hadidi A (1994) Simple and rapid preparation of infected plant tissue extracts for PCR amplification of virus, viroid, and MLO nucleic acids. J Virol Methods 49:295–304PubMedCrossRefGoogle Scholar
  4. Li S-F, Hataya T, Furuta K, Horita H, Sano T, Shikata E (1997) Occurrence of chrysanthemum stunt disease in Hokkaido and detection of chrysanthemum stunt viroid by electrophoresis and hybridization (in Japanese). Annu Rep Soc Plant Prot North Jpn 48:113–117Google Scholar
  5. Nakashima A, Hosokawa M, Maeda S, Yazawa S (2007) Natural infection of Chrysanthemum stunt viroid in dahlia plants. J Gen Plant Pathol 73:225–227CrossRefGoogle Scholar
  6. Sano T, Yoshida H, Goshono M, Monma T, Kawasaki H, Ishizaki K (2004) Characterization of a new viroid strain from hops: evidence for viroid speciation by isolation in different host species. J Gen Plant Pathol 70:181–187CrossRefGoogle Scholar
  7. Schumacher J, Randles JW, Riesner D (1983) A two-dimensional electrophoretic technique for the detection of circular viroids and virusoids. Anal Biochem 135:288–295PubMedCrossRefGoogle Scholar
  8. Weidemann H-L, Buchta U (1998) A simple and rapid method for the detection of potato spindle tuber viroid (PSTVd) by RT-PCR. Potato Res 41:1–8CrossRefGoogle Scholar
  9. Zuker M (2003) Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Res 31:3406–3415PubMedCrossRefGoogle Scholar

Copyright information

© The Phytopathological Society of Japan and Springer 2011

Authors and Affiliations

  • Taro Tsushima
    • 1
  • Sakiko Murakami
    • 1
  • Hirotsugu Ito
    • 1
  • Ying-Hong He
    • 1
  • Adkar Purushothama Charith Raj
    • 1
  • Teruo Sano
    • 1
    Email author
  1. 1.Faculty of Agriculture and Life ScienceHirosaki UniversityHirosakiJapan

Personalised recommendations