Skip to main content
SpringerLink
Log in

Improved method to induce sporulation of Colletotrichum gloeosporioides, causal fungus of grape ripe rot

  • Technique
  • Published:
Journal of General Plant Pathology Aims and scope Submit manuscript

Abstract

Colletotrichumgloeosporioides and C. acutatum are causal agents of grape ripe rot, but with available methods, sporulation of C.gloeosporioides on plate media has been unstable and inferior to that of C. acutatum. To facilitate studies on C. gloeosporioides, I developed an improved method to induce conidiation of this fungus. Isolates of C. gloeosporioides were pre-cultured in potato dextrose broth for 1 week, then pulverized in whole broth. The homogenate was then spread on diluted oatmeal agar (15–20% commercial oatmeal agar medium, 1.5% agar) plates. After the plates were cultured at 25°C under continuous light for another week, the C. gloeosporioides isolates sporulated stably on the plate medium.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Baxter LW Jr, Fagan SG (1974) A simplified method of inducing asexual sporulation in Glomerella cingulata. Plant Dis Rep 58:300–303

    Google Scholar 

  • Cooke BM, Jones DG (1970) The effect of near-ultraviolet irradiation and agar medium on the sporulation of Septoria nodorum and S. tritici. Trans Br Mycol Soc 54:221–226

    Article  Google Scholar 

  • Fukaya M (1995) Ripe rot. In: Ohata K, Araki T, Kiso A, Kudo A, Takahashi H (eds) Methods for isolation, cultivation, inoculation of plant pathogens (in Japanese). Japan Plant Protection Association, Tokyo, Japan, pp 257–258

  • Johnston PR, Jones D (1997) Relationships among Colletotrichum isolates from fruit-rots assessed using rDNA sequences. Mycologia 89:420–430

    Article  CAS  Google Scholar 

  • Kaiser WJ (1973) Factors affecting growth, sporulation, pathogenicity, and survival of Ascochyta rabiei. Mycologia 65:444–457

    Article  PubMed  CAS  Google Scholar 

  • Miller SB, Baxter LW Jr (1970) Some factors influencing asexual sporulation in a strain of Glomerella cingulata pathogenic to camellias. Phytopathology 60:743–744

    Article  Google Scholar 

  • Namai T, Yamanaka S (1983) A component contained in oatmeal that favors the sporulation of rice blast fungus, Pyricularia oryzae Cavara (in Japanese with English summary). Ann Phytopathol Soc Jpn 49:274–276

    CAS  Google Scholar 

  • Namai T, Yamanaka S (1985) Studies on the sporulation of rice blast fungus, Pyricularia oryzae Cavara. IV. Effect of hyphal age and culture media on sporulation. Tohoku J Agric Res 35:69–79

    Google Scholar 

  • Noguchi Y, Mochizuki T, Yamakawa O (1994) Petiole dip inoculation is a convenient method for screening strawberry for resistance to anthracnose caused by Colletotrichum fragariae (in Japanese). Bull Natl Res Inst Veg Ornam Plants Tea Jpn A9:13–26

    Google Scholar 

  • Ozoe S, Takuda T, Hirosawa T (1972) Studies on the etiology and the control of the ripe-rot of grapes. I. The primary occurrence of the disease and the chemical control in dormant period of grapes (in Japanese with English summary). Bull Shimane Agric Exp Stn 10:120–158

    Google Scholar 

  • Shiraishi M, Yamada M, Mitani N, Ueno T, Nakaune R, Nakano M (2006) Rapid screening assay for ripe rot resistance in grape cultivars. J Jpn Soc Hort Sci 75:264–266

    Article  CAS  Google Scholar 

  • Shiraishi M, Koide M, Itamura H, Yamada M, Mitani N, Ueno T, Nakaune R, Nakano M (2007) Screening for resistance to ripe rot caused by Colletotrichum acutatum in grape germplasm. Vitis 46:196–200

    Google Scholar 

  • Sutton BC (1992) The genus Glomerella and its anamorph Colletotrichum. In: Bailey JA, Jeger MJ (eds) Colletotrichum biology, pathology and control. CAB International, Wallingford, pp 1–26

    Google Scholar 

  • Yamamoto J, Sato T, Tomioka K (1999) Occurrence of ripe rot of grapes (Vitis vinifera L.) caused by Colletotrichum acutatum Simmonds ex Simmonds (in Japanese). Ann Phytopathol Soc Jpn 65:83–86

    Google Scholar 

Download references

Acknowledgments

I thank Drs. Mikio Shiraishi (Fukuoka Agricultural Research Center), Yukihiro Kunugi (Yamanashi Fruit Tree Experiment Station) and Kenichi Kondo (Nagano Fruit Tree Experiment Station) for valuable advice and kindly supplying fungal isolates.

Author information

Authors and Affiliations

  1. Grape and Persimmon Research Station, National Institute of Fruit Tree Science, National Agriculture and Food Research Organization, Akitsu 301-2, Higashihiroshima, Hiroshima, 739-2494, Japan

    Koichi Suzaki

Authors
  1. Koichi Suzaki
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Koichi Suzaki.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Suzaki, K. Improved method to induce sporulation of Colletotrichum gloeosporioides, causal fungus of grape ripe rot. J Gen Plant Pathol 77, 81–84 (2011). https://doi.org/10.1007/s10327-011-0296-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10327-011-0296-z

Keywords

Search

Navigation