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The first report of tomato foot rot caused by Rhizoctonia solani AG-3 PT and AG-2-Nt and its host range and molecular characterization

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Abstract

Foot rot of mature tomato plants was found in four cities of Hokkaido, Japan, from 2004 to 2007. Six of eight isolates obtained from damaged tissues were identified as Rhizoctonia solani anastomosis group (AG)-3, and the remaining two isolates belonged to AG-2-1. We compared these isolates with nine reference isolates including the different subgroups in AG-3 (PT, TB and TM) and AG-2-Nt (pathogen of tobacco leaf spot) within AG-2-1 in terms of pathogenicity to tomato, tobacco and potato. All eight isolates caused foot rot on tomato. The six AG-3 isolates caused stem rot on young potato plants. While, all reference isolates of AG-3 PT causing stem rot of young potato plants incited foot rot on tomato. The two AG-2-1 isolates and an AG-2-Nt reference isolate caused severe leaf spot on tobacco leaves. The sequences of rDNA- ITS region and rDNA-IGS1 region of the AG-3 isolates showed high similarity to that of AG-3 PT isolates. Phylogenetic tree based on ITS and IGS1 regions of rDNA indicated that the AG-2-1 isolates from tomato formed a single clade with AG-2-Nt isolates and that they were separate from Japanese AG-2-1 isolates (culture type II). Pathogenicity tests and DNA sequence evaluation of the causal fungi revealed that the present isolates of AG-3 and AG-2-1 belonged to AG-3 PT and AG-2-Nt, respectively. This is the first report of tomato foot rot caused by R. solani in Japan.

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Acknowledgments

For help with sampling for tomato isolates of R. solani, we thank T. Komatsu (Kamikawa Agricultural Experiment Station, Japan). We also thank Dr. D. E. Carling (University of Alaska, USA), Dr. R. Nicoletti (Tobacco Research Institute, Italy), Dr. A. Sayama (Akita Prefectural Agriculture, Forestry and Fisheries Research Center, Japan) and S. Ikeda (Tokachi Agricultural Experiment Station, Japan) for providing reference isolates of R. solani. Special thanks are due to Dr. N. Matsumoto (Hokkaido National Agricultural Research Center, Japan) for valuable comments and English correction of the manuscript.

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Authors and Affiliations

  1. Dohnan Agricultural Experiment Station, Agricultural Research Department, Hokkaido Research Organization, Hokuto, Hokkaido, 041-1201, Japan

    Tomoo Misawa

  2. Health Sciences University of Hokkaido, Ishikari-Tobetsu, Hokkaido, 061-0293, Japan

    Shiro Kuninaga

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  1. Tomoo Misawa
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  2. Shiro Kuninaga
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Correspondence to Tomoo Misawa.

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The nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank databases as accessions AB547345-547397.

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Misawa, T., Kuninaga, S. The first report of tomato foot rot caused by Rhizoctonia solani AG-3 PT and AG-2-Nt and its host range and molecular characterization. J Gen Plant Pathol 76, 310–319 (2010). https://doi.org/10.1007/s10327-010-0261-2

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