Abstract
Two isolates of Pythium nunn, newly recorded in Japan, were obtained from soils in Nagano and Fukuoka prefectures, characterized, and tested for antagonistic activity against P. ultimum var. ultimum. The morphology of both isolates corresponded with those of the original description of P. nunn. The rDNA-ITS sequences of the two isolates were identical to each other and had a high similarity with the sequences of the type strain of P. nunn. The two P. nunn isolates were mycoparasitic toward P. ultimum var. ultimum and suppressed damping off of cucumber seedlings caused by the pathogen at an early stage of plant growth.



Similar content being viewed by others
References
Adams PB (1990) The potential of mycoparasites for biological control of plant diseases. Annu Rev Phytophthol 28:59–72
Fang JG, Tsao PH (1995) Evaluation of Pythium nunn as a potential biocontrol agent against Phytophthora root rots of azalea and sweet orange. Phytopathology 85:29–36
Kida K, Tojo M, Yano K, Kotani S (2007) First report of Pythium ultimum var. ultimum causing damping-off on okra in Japan. Plant Pathol 56:1042
Kinoshita T, Ichitani T, Okumura T (1994) Materials for Pythium flora of Japan VIII. Two species of Pythium: P. pyrilobum and P. oligandrum. Mycoscience 35:191–198
Kurokawa K, Tojo M (2010) First record of Pythium grandisporangium in Japan. Mycoscience. doi:10.1007/s10267-010-0041-z
Laing SAK, Deacon JW (1991) Video microscopical comparison of mycoparasitism by Pythium oligandrum, P. nunn and an unnamed Pythium species. Mycol Res 95:469–479
Lévesque CA, de Cock AWAM (2004) Molecular phylogeny and taxonomy of the genus Pythium. Mycol Res 108:1363–1383
Lifshitz R, Dupler M, Elad Y, Baker R (1984a) Hyphal interactions between a mycoparasite, Pythium nunn and several soil fungi. Can J Microbiol 30:1482–1487
Lifshitz R, Sneh B, Baker R (1984b) Soil suppressiveness to a plant pathogenic Pythium species. Phytopathology 74:1054–1061
Lifshitz R, Stanghellini ME, Baker R (1984c) A new species of Pythium isolated from soil in Colorado. Mycotaxon 20:373–379
Martin FN (1992) Pythium. In: Singleton LL, Mihail JD, Rush CM (eds) Methods for research on soilborne phytopathogenic fungi. APS Press, St. Paul, pp 39–49
Martin FN, Hancock JG (1987) The uses of Pythium oligandrum for biological control of preemergence damping-off caused by P. ultimum. Phytopathology 77:1013–1020
McQuilken MP, Whipps JM, Cooke RC (1992) Use of oospore formulations of Pythium oligandrum for biological control of Pythium damping-off in cress. Phytopathology 135:125–134
Paulitz TC, Baker R (1987a) Biological control of Pythium damping-off of cucumbers with Pythium nunn: population dynamics and disease suppression. Phytopathology 77:335–340
Paulitz TC, Baker R (1987b) Biological control of Pythium damping-off of cucumbers with Pythium nunn: influence of soil environment and organic amendments. Phytopathology 77:341–346
Paulitz TC, Baker R (1988a) The formation of secondary sporangia by Pythium ultimum: the influence of organic amendments and Pythium nunn. Soil Biol Biochem 20:151–156
Paulitz TC, Baker R (1988b) Interactions between Pythium nunn and Pythium ultimum on bean leaves. Can J Microbiol 34:947–951
Paulitz TC, Windham M, Baker R (1985) Pythium nunn—a potential biological control agent. Phytopathology 75:1326–1327
Paulitz TC, Ahmad JS, Baker R (1990) Integration of Pythium nunn and Trichoderma harzianum isolate T-95 for the biological control of Pythium damping-off of cucumber. Plant Soil 121:243–250
Perneel M, Tambong JT, Adiobo A, Foloren C, Saborío F, Lévesque A, Höfte M (2006) Intraspecific variability of Pythium myriotylum isolated from cocoyam and other host crops. Mycol Res 110:583–593
Takenaka S, Sekiguchi H, Nakaho K, Tojo M, Masunaka A, Takahashi H (2008) Colonization of Pythium oligandrum in the tomato rhizosphere for biological control of bacterial wilt disease analyzed by real-time PCR and confocal laser-scanning microscopy. Phytopathology 98:187–195
Tojo M, Nakazono E, Tsushima S, Morikawa T, Matsumoto N (1998) Characterization of two morphological groups of isolates of Pythium ultimum var. ultimum in a vegetable field. Mycoscience 39:135–144
Uzuhashi S, Tojo M, Kobayashi S, Tokura K, Kakishima M (2008) First records of Pythium aquatile and P. macrosporum isolated from soils in Japan. Mycoscience 49:276–279
Uzuhashi S, Tojo M, Kobayashi S, Kakishima M (2009) Pythium apinafurcum: its morphology, molecular phylogeny, and infectivity for plants. Mycoscience 50:281–290
van der Plaats-Niterink AJ (1981) Monograph of the genus Pythium. Stud Mycol 21:1–242
Watanabe T (1981) Distribution and populations of Pythium species in the northern and southern parts of Japan. Ann Phytopathol Soc Jpn 47:449–456
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Kobayashi, S., Uzuhashi, S., Tojo, M. et al. Characterization of Pythium nunn newly recorded in Japan and its antagonistic activity against P. ultimum var. ultimum . J Gen Plant Pathol 76, 278–283 (2010). https://doi.org/10.1007/s10327-010-0244-3
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10327-010-0244-3


