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Journal of General Plant Pathology

, Volume 76, Issue 3, pp 188–195 | Cite as

Exogenous coronatine, but not coronafacic acid or methyl jasmonate, restores the disease phenotype of a coronatine-defective mutant of Pseudomonas syringae pv. tomato on tomato seedlings

  • Yasuhiro Ishiga
  • Srinivasa Rao UppalapatiEmail author
  • Takako Ishiga
  • Carol L. BenderEmail author
Bacterial and Phytoplasma Diseases

Abstract

Coronatine (COR) functions as a virulence factor in the interaction of Pseudomonas syringae pv. tomato strain DC3000 with tomato and Arabidopsis. COR consists of two moieties, coronafacic acid (CFA) and coronamic acid (CMA). Both COR and CFA function as structural and functional analogues of jasmonic acid (JA) and related signaling compounds such as methyl jasmonate (MeJA) and JA-isoleucine (JA-Ile). The precise function of COR and whether MeJA functions as an analogue of COR in disease development are not known. In this study, we analyzed whether the COR-defective mutant DB29, which is a CFA CMA mutant of DC3000, could be complemented for virulence via the exogenous application of COR, CFA, or MeJA. When tomato seedlings were inoculated with DB29 and supplemented with exogenous COR, the DB29 population multiplied in tomato seedlings and induced disease phenotypes similar to wild-type DC3000. Although the addition of exogenous MeJA or CFA enhanced the multiplication of DB29, wild-type disease phenotypes could not be restored with these compounds. Furthermore, inoculation of DB29 along with exogenous COR, but not MeJA or CFA, suppressed the expression of defense-related genes and increased the accumulation of reactive oxygen species, which are associated with severe chlorosis. Taken together, our results suggest that although COR targets the jasmonate pathway by mimicking JA, the function of COR in disease development is distinctly different from MeJA or CFA.

Keywords

Bacterial speck Coronatine Coronafacic acid Coronamic acid Jasmonates Pseudomonas syringae pv. tomato Solanum lycopersicum Tomato 

Notes

Acknowledgments

This study was funded in part by National Science Foundation grant IBN-0620469 and the Oklahoma Agricultural Experiment Station (C. L. Bender). Y. Ishiga was supported by a postdoctoral fellowship from the Japan Society for the Promotion of Science (JSPS). The OSU Microarray Facility was supported by grants from NSF (EOS-0132534) and NIH (1P20RR16478-02 and 5P20RR15564-03). The authors also thank Dr. Kiran Mysore for providing some of the resources required to complete this work.

Supplementary material

10327_2010_228_MOESM1_ESM.pptx (39 mb)
Supplementary material 1 (PPTX 39929 kb)

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Copyright information

© The Phytopathological Society of Japan and Springer 2010

Authors and Affiliations

  1. 1.Department of Entomology and Plant PathologyOklahoma State UniversityStillwaterUSA
  2. 2.Plant Biology DivisionThe Samuel Roberts Noble FoundationArdmoreUSA

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