Genetic diversity and pathogenicity of Fusarium oxysporum isolated from wilted Welsh onion in Japan
- 271 Downloads
Thirty isolates of Fusarium oxysporum from wilted Welsh onion plants were examined for their diversity in nucleotide sequences of the ribosomal DNA (rDNA) intergenic spacer (IGS) region and for pathogenicity with regard to five Welsh onion cultivars. Phylogenetic analysis based on the IGS sequences revealed polyphyletic origins of the isolates and a relationship between phylogeny and pathogenicity; low virulence isolates differed genetically from those with high and moderate virulence. Mating type analysis revealed that all F. oxysporum isolates were MAT1-1 idiomorphs, suggesting that the pathogens may be clonal in the fields examined.
KeywordsAllium fistulosum Fusarium wilt Mating type Phylogeny
We thank Mr. Masataka Funahashi, Nissan Seed, Nishinomiya, Japan, for providing wilted Welsh onion samples. This work was supported in part by Nissan Seed Foundation.
- Enya J, Togawa M, Takeuchi T, Yoshida S, Tsushima S, Arie T, Sakai T (2008) Biological and phylogenetic characterization of Fusarium oxysporum complex, which causes yellows on Brassica spp., and proposal of F. oxysporum f. sp. rapae, a novel forma specialis pathogenic on B. rapa in Japan. Phytopathology 98:475–483PubMedCrossRefGoogle Scholar
- Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98Google Scholar
- Kashima R, Dissanayake C, Dogo M, Funahashi M, Shigyo M, Ito S (2007) Fusarium species isolated from bunching onion showing wilt symptoms (in Japanese). Hort Res (Japan) 6(Suppl 2):267Google Scholar
- Kawabe M, Kobayashi Y, Okada G, Yamaguchi I, Teraoka T, Arie T (2005) Three evolutionary lineages of tomato wilt pathogen, Fusarium oxysporum f. sp. lycopersici, based on sequences of IGS, MAT1 and pg1, are each composed of isolates of a single mating type and a single or closely related vegetative compatibility group. J Gen Plant Pathol 71:263–272CrossRefGoogle Scholar
- Kodama F (1977) Fusarium wilt disease of Welsh onion caused by Fusarium oxysporum (Abstract in Japanese). Ann Phytopathol Soc Jpn 43:340Google Scholar
- Kodama F (1983) Studies on basal rot of onion caused by Fusarium oxysporum f. sp. cepae and its control (in Japanese with English summary). Rep Hokkaido Pref Agric Expt Stn 39:1–65Google Scholar
- Komada H (1975) Development of a selective medium for quantitative isolation of Fusarium oxysporum from natural soils. Rev Plant Prot Res 8:114–125Google Scholar
- Nelson PE, Toussoun TA, Marasas WFO (1983) Fusarium species: an illustrated manual for identification. The Pennsylvania State University Press, University Park, PAGoogle Scholar
- Takakuwa M, Ishizaka N, Kodama F, Saito I (1977) Host range of Fusarium oxysporum f. sp. cepae, causal fungus of Fusarium basal rot of onion (in Japanese with English summary). Ann Phytopathol Soc Jpn 43:479–481Google Scholar
- Takeuchi T, Sinohara S, Nagai Y (1983) Survey on occurrence of Fusarium wilt of Welsh onion and its control. Bull Chiba Agric Exp Stn 24:1–6Google Scholar
- Yoo SJ, Wakanabe H, Kobayashi K, Ogoshi A, Kodama F (1993) Vegetative compatibility grouping of formae speciales of Fusarium oxysporum pathogenic to Liliaceae. Ann Phytopathol Soc Jpn 59:3–9Google Scholar