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Morphological and molecular characterization of Oidium subgenus Reticuloidium (powdery mildew) newly occurred on cucumber in Japan

  • Fungal Diseases
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Abstract

In 2002, a powdery mildew with catenate conidia lacking fibrosin bodies was found on cucumber in a greenhouse in Kanagawa Prefecture, Japan. Morphological observation revealed that the fungus belongs to Oidium subgenus Reticuloidium, anamorph of the genus Golovinomyces. Molecular phylogenetic analyses of the nucleotide sequences of the rDNA ITS regions and D1/D2 domains of the 28S rDNA indicated that the fungus belongs to the clade of G. orontii with other Golovinomyces fungi from a wide range of host plants, suggesting that the fungus was newly transported from abroad. Because there has been no prior report of cucumber powdery mildew caused by Reticuloidium, further research on the physiology, epidemiology, control and resistant cucumber varieties is required.

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References

  • Abiko K (1978) Studies on the specialization of parasitism of Sphaerotheca fuliginea (Schlecht.) Pollacci I. Powdery mildew fungi parasitic on cucurbits, eggplant, edible burdock and Japanese butterbur. Ann Phytopathol Soc Jpn 44:612–618

    Google Scholar 

  • Abiko K (1982a) Studies on the specialization of parasitism of Sphaerotheca fuliginea (Schlecht.) Pollacci II. Powdery mildew fungi on flowering plants. Bull Veg Ornam Crops Res Stn Ser A 10:57–62

    Google Scholar 

  • Abiko K (1982b) Studies on the specialization of parasitism of Sphaerotheca fuliginea (Schlecht.) Pollacci II. Powdery mildew fungi parasitic on weeds. Bull Veg Ornam Crops Res Stn Ser A 10:63–67

    Google Scholar 

  • Amano K (1986) Host range and geographical distribution of the powdery mildew fungi. Japan Scientific Societies Press, Tokyo

    Google Scholar 

  • Bardin M, Carlier J, Nicot PC (1999) Genetic differentiation in the French population of Erysiphe cichoracearum, a causal agent of powdery mildew of cucurbits. Plant Pathol 48:531–540

    Article  Google Scholar 

  • Blumer S (1952) Beiträge zur Spezialisation der Erysipheen (in German). Ber Schweiz Bot Ges 62:384–401

    Google Scholar 

  • Blumer S (1967) Echte Mehltaupilze (Erysiphaceae) (in German). Gustav Fischer, Jena

    Google Scholar 

  • Braun U (1980) Morphological studies in the genus Oidium. Flora 170:77–90

    Google Scholar 

  • Braun U (1987) A monograph of the Erysiphales (powdery mildews). Beih Nova Hedwig 89:1–700

    Google Scholar 

  • Braun U (1999) Some critical notes on the classification and generic concept of the Erysiphaceae. Schlechtendalia 3:49–55

    Google Scholar 

  • Braun U, Takamatsu S (2000) Phylogeny of Erysiphe, Microsphaera, Uncinula (Erysipheae) and Cystotheca, Podosphaera, Sphaerotheca (Cystotheceae) inferred from rDNA ITS sequences—some taxonomic consequences. Schlechtendalia 4:1–33

    Google Scholar 

  • Braun U, Shishkoff N, Takamatsu S (2001) Phylogeny of Podosphaera sect. Sphaerotheca subsect. Magnicellulatae (Sphaerotheca fuliginea auct. s. lat.) inferred from rDNA ITS sequences—a taxonomic interpretation. Schlechtendalia 7:45–52

    Google Scholar 

  • Braun U, Cook RTA, Inman AJ, Shin HD (2002) The taxonomy of the powdery mildew fungi. In: Bélanger R, Bushnell WR, Dik AJ, Carver TLW (eds) The powdery mildews: a comprehensive treatise. APS Press, St. Paul, pp 13–55

    Google Scholar 

  • Endo T (1989) Studies on the life-cycle of cucurbit powdery mildew fungus Sphaerotheca fuliginea (Schlecht.) Poll (in Japanese with English summary). Spec Bull Fukushima Pref Agr Exp Stn 5:1–106

    Google Scholar 

  • Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791

    Article  Google Scholar 

  • Garibaldi A, Bertetti D, Minerdi D, Gullino ML (2007) First report of powdery mildew caused by Golovinomyces orontii (Erysiphe orontii) on Lamium galeobdolon in Italy. Plant Dis 91:635

    Article  Google Scholar 

  • Hammarlund C (1945) Beiträge zur Revision einiger imperfekter Mehltau-Arten. Erysiphe polyphaga nov. sp. (in German). Bot Not 1945:101–108

    Google Scholar 

  • Heluta VP (1988) Phylogenetic connections among genera of powdery mildew fungi and some questions of systematics of Erysiphales. Biol J Arm 41:351–358

    Google Scholar 

  • Hirata K (1942) On the shape of the germ tubes of Erysiphaceae (in Japanese). Bull Chiba Coll Hort 5:34–49

    Google Scholar 

  • Hirata K (1955) On the shape of the germ tubes of Erysiphaceae II (in Japanese). Bull Fac Agric Niigata Univ 7:24–36

    Google Scholar 

  • Hirata T, Takamatsu S (1996) Nucleotide sequence diversity of rDNA internal transcribed spacers extracted from conidia and cleistothecia of several powdery mildew fungi. Mycoscience 37:283–288

    Article  Google Scholar 

  • Hoshi H, Sato Y, Horie H (2007) Occurrence of zinnia and Jerusalem artichoke powdery mildew by Oidium subgenus Reticuloidium, and host range of the subgenus of powdery mildew on several plant species occurred at Tokyo (abstract in Japanese). Ann Phytopathol Soc Jpn 73:182

    Google Scholar 

  • Hosoya K, Narisawa K, Pitrat M, Ezura H (1999) Race identification in powdery mildew (Sphaerotheca fuliginea)on melon (Cucumis melo L.) in Japan. Plant Breeding 118:259–262

    Article  Google Scholar 

  • Hosoya K, Kuzuya M, Murakami T, Kato K, Narisawa K, Ezura H (2000) Impact of the resistant melon cultivars on Sphaerotheca fuliginea. Plant Breeding 119:286–288

    Article  Google Scholar 

  • Jahn M, Munger HM, McCreight JD (2002) Breeding cucurbit crops for powdery mildew resistance. In: Bélanger R, Bushnell WR, Dik AJ, Carver TLW (eds) The powdery mildews: a comprehensive treatise. APS Press, St Paul, pp 239–248

    Google Scholar 

  • Kuzuya M, Hosoya K, Yashiro K, Tomita K, Ezura H (2003) Powdery mildew (Sphaerotheca fuliginea) resistance in melon is selectable at the haploid level. J Exp Bot 54:1069–1074

    Article  PubMed  CAS  Google Scholar 

  • Kuzuya M, Yashiro K, Tomita K, Ezura H (2006) Powdery mildew (Podosphaera xanthii) resistance in melon is categorized into two types based on inhibition of the infection processes. J Exp Bot 57:2093–2100

    Article  PubMed  CAS  Google Scholar 

  • Matsuda S, Takamatsu S (2003) Evolution of host–parasite relationship of Golovinomyces (Ascomycete: Erysiphales) inferred from nuclear rDNA sequences. Mol Phylogenet Evol 27:314–327

    Article  PubMed  CAS  Google Scholar 

  • Mohamed YF, Bardin M, Nicot PC, Pitrat M (1995) Causal agents of powdery mildew of cucurbits in Sudan. Plant Dis 79:634–636

    Google Scholar 

  • Mori Y, Sato Y, Takamatsu S (2000) Evolutionary analysis of the powdery mildew fungi using nucleotide sequences of the nuclear ribosomal DNA. Mycologia 92:74–93

    Article  CAS  Google Scholar 

  • Nomura Y (1997) Taxonomical study of Erysiphaceae of Japan (in Japanese with English summary). Yokendo, Tokyo

    Google Scholar 

  • Phytopathological Society of Japan (ed) (2000) Common names of plant diseases in Japan (in Japanese). Japan Plant Protection Association, Tokyo

  • Saito M, Kurata M (1975) Occurrence of Leveillula taurica on cucumber, okra and eggplant (abstract in Japanese). Ann Phytophathol Soc Jpn 41:269

    Google Scholar 

  • Salmon E (1900) A monograph of the Erysiphaceae. Mem Torrey Bot Club 9:1–292

    Google Scholar 

  • Sato Y, Nakamura T, Takamatsu S, Morikawa T, Chikuo Y (1996) Powdery mildew fungi newly found on Datura stramonium and Cucumis sativus in Japan (abstract in Japanese). Ann Phytophathol Soc Jpn 62:630

    Google Scholar 

  • Shin HD (2000) The Erysiphaceae of Korea. National Institute of Agricultural Science & Technology, Suwon

    Google Scholar 

  • Sitterly WR (1978) Powdery mildews of cucurbits. In: Spencer DM (ed) The powdery mildews. APS Press, St. Paul, pp 359–379

    Google Scholar 

  • Swofford DL (2001) PAUP*: phylogenetic analysis using parsimony (*and other methods), version 4.0b8. Sinauer, Sunderland

    Google Scholar 

  • Sz Nagy G (1970) Die Identifizierung des Mehltaus der Kürbisgewächse auf Grund der Konidienmerkmale (in German with English summary). Acta Phytopathol Hung 5:231–248

    Google Scholar 

  • Sz Nagy G (1972) Studies on powdery mildews of cucurbits. I. Host range and maintenance of Sphaerotheca fuliginea and Erysiphe sp. under laboratory and glasshouse conditions. Acta Phytopathol Hung 7:415–420

    Google Scholar 

  • Sz Nagy G (1976) Studies on powdery mildews of cucurbits II. Life cycle and epidemiology of Erysiphe cichoracearum and Sphaerotheca fuliginea. Acta Phytopathol Hung 7:415–420

    Google Scholar 

  • Tanda S, Suga R (2002) Powdery mildews occurred on horseradish and five economic plants, and their causal fungi (in Japanese with English summary). J Agri Sci Tokyo Univ of Agric 47:141–152

    Google Scholar 

  • Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The Clustal_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 24:4876–4882

    Article  Google Scholar 

  • Uchida K, So K, Niinomi S, Takamatsu S (2002) Morphological characteristics and parasitism of powdery mildew on Lamium amplexicaule (abstract in Japanese). Ann Phytophathol Soc Jpn 68:189

    Google Scholar 

  • Vakalounakis DJ, Klironomou E (1995) Race and mating type identification of powdery mildew on cucurbits in Greece. Plant Pathol 44:1033–1038

    Article  Google Scholar 

  • Vakalounakis DJ, Klironomou E, Papadakis A (1994) Species spectrum, host range and distribution of powdery mildews on Cucurbitaceae in Crete. Plant Pathol 43:813–818

    Article  Google Scholar 

  • Walsh PS, Metzger DA, Higuchi R (1991) Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. BioTechniques 10:506–513

    PubMed  CAS  Google Scholar 

Download references

Acknowledgments

We are grateful to Dr. Hideo Ishii (National Institute for Agro-Environmental Sciences, Japan) for his helpful advice during this study and for critically reading the manuscript. This work was partially supported by a Grant-in-Aid for Scientific Research (C) (No. 18580046) from the Ministry of Education, Science, Sports and Culture of Japan.

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Authors and Affiliations

  1. ZEN-NOH Agricultural R&D Center, 5-5-1 Higashi-Yahata, Hiratsuka, 254-0016, Japan

    Keiko Uchida & Kazuhiro So

  2. Graduate School of Bioresources, Mie University, 1577 Kurima-Machiya, Tsu, 514-8507, Japan

    Susumu Takamatsu & Sanae Matsuda

  3. Department of Liberal Arts and Sciences, Faculty of Engineering, Toyama Prefectural University, 5180 Kurokawa, Imizu, 939-0398, Japan

    Yukio Sato

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  1. Keiko Uchida
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  2. Susumu Takamatsu
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  3. Sanae Matsuda
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  4. Kazuhiro So
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  5. Yukio Sato
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Correspondence to Keiko Uchida.

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Uchida, K., Takamatsu, S., Matsuda, S. et al. Morphological and molecular characterization of Oidium subgenus Reticuloidium (powdery mildew) newly occurred on cucumber in Japan. J Gen Plant Pathol 75, 92–100 (2009). https://doi.org/10.1007/s10327-009-0146-4

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