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Journal of General Plant Pathology

, Volume 74, Issue 2, pp 151–155 | Cite as

Rapid and sensitive detection of “Candidatus Liberibacter asiaticus” by cycleave isothermal and chimeric primer-initiated amplification of nucleic acids

  • Naoya Urasaki
  • Shinji KawanoEmail author
  • Hiroyuki Mukai
  • Takashi Uemori
  • Osamu Takeda
  • Teruo Sano
Bacterial and Phytoplasma Diseases

Abstract

We developed a detection method for “Candidatus Liberibacter asiaticus”, causal agent of citrus huanglongbing, using isothermal and chimeric primer-initiated amplification of nucleic acids combined with cycling probe technology (Cycleave ICAN). With Cycleave ICAN, the reaction was done in one tube in 1 h without the need for electrophoresis, and false positives were not generated. In addition, Cycleave ICAN method was more sensitive than the conventional PCR method. Cycleave ICAN helps shorten the time for the large-scale detection needed to manage huanglongbing.

Keywords

Citrus huanglongbing Candidatus Liberibacter asiaticus” Citrus psyllid Cycleave isothermal and chimeric primer-initiated amplification of nucleic acids (Cycleave ICAN) Cycling probe technology 

Notes

Acknowledgments

We thank Mr. Tsukasa Uehara, Mr. Masaya Maeda, Ms. Eriko Kawatake and Ms. Yuko Murayama for their technical support. We especially thank Mr. Tuyoshi Ooishi for providing citrus psyllids. This research (No.1666) was supported by a grant from Agriculture, Forestry and Fisheries Research Council.

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Copyright information

© The Phytopathological Society of Japan and Springer 2008

Authors and Affiliations

  • Naoya Urasaki
    • 1
  • Shinji Kawano
    • 1
    Email author
  • Hiroyuki Mukai
    • 2
  • Takashi Uemori
    • 2
  • Osamu Takeda
    • 2
  • Teruo Sano
    • 3
  1. 1.Okinawa Prefectural Agricultural Research CenterItoman, OkinawaJapan
  2. 2.Products Development Center, TAKARA BIO INC.KusatsuJapan
  3. 3.Hirosaki UniversityHirosakiJapan

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