Abstract
Twelve isolates of Japanese Phytophthora infestans, which differed from the major genotypes US-1, JP-1, JP-2, and JP-3, were analyzed for RG57 fingerprints, mtDNA haplotypes, two allozyme genotypes, and mating types. Genotypes JP-1.1, JP-2.1, JP-2.2, JP-3.1, and JP-4 were newly defined. JP-1.1 and JP-2.1 were isolated discontinuously from potato fields in several years, and JP-1.1 was found in Hokkaido and Kagoshima. These results show that some minor genotypes can overwinter and disperse from their original site.
Similar content being viewed by others
References
Akino S, Kato M, Gotoh K, Naito S, Ogoshi A (2005) Genetic relationships between the dominant genotypes of Phytophthora infestans in Hokkaido, Japan. J Gen Plant Pathol 71:200–203
Caten CE, Jinks JL (1968) Spontaneous variability of single isolates of Phytophthora infestans. I. Cultural variation. Can J Bot 46:329–348
Drenth A, Tas ICQ, Govers F (1994) DNA fingerprinting uncovers a new sexually reproducing population of Phytophthora infestans in the Netherlands. Eur J Plant Pathol 100:97–107
Forbes GA, Goodwin SB, Drenth A, Oyarzun P, Ordoñez ME, Fry WE (1998) A global marker database for Phytophthora infestans. Plant Dis 82:811–818
Goodwin SB, Drenth A, Fry WE (1992) Cloning and genetic analyses of two highly polymorphic, moderately repetitive nuclear DNAs from Phytophthora infestans. Curr Genet 22:107–115
Goodwin SB, Cohen BA, Deahl KL, Fry WE (1994a) Migration from northern Mexico as the probable cause of recent genetic changes in populations of Phytophthora infestans in the United States and Canada. Phytopathology 84:553–558
Goodwin SB, Cohen BA, Fry WE (1994b) Panglobal distribution of a single clonal lineage of the Irish potato famine fungus. Proc Natl Acad Sci USA 91:11591–11595
Goodwin SB, Schneider RE, Fry WE (1995) Use of cellulose–acetate electrophoresis for rapid identification of allozyme genotypes of Phytophthora infestans. Plant Dis 79:1181–1185
Gotoh K, Akino S, Maeda A, Kondo N, Naito S, Kato M, Ogoshi A (2005) Characterization of some Asian isolates of Phytophthora infestans. Plant Pathol 54:733–739
Griffith GW, Shaw DS (1998) Polymorphisms in Phytophthora infestans: four mitochondrial haplotypes are detected after PCR amplification of DNA from pure cultures or from host lesions. Appl Environ Microbiol 64:4007–4014
Kato M (2001) Replacement of genotype of Phytophthora infestans and factors for the replacement (in Japanese). Plant Prot 55:467–470
Koh YJ, Goodwin SB, Dyer AT, Cohen BA, Ogoshi A, Sato N, Fry WE (1994) Migrations and displacements of Phytophthora infestans populations in East Asian countries. Phytopathology 84: 922–927
Mosa AA, Kobayashi K, Ogoshi A, Kato M, Sato N (1993) Isoenzyme polymorphism and segregation in isolates of Phytophthora infestans from Japan. Plant Pathol 42:26–34
Sujkowski LS, Goodwin SB, Dyer AT, Fry WE (1994) Increased genotypic diversity via migration and possible occurrence of sexual reproduction of Phytophthora infestans in Poland. Phytopathology 84:201–207
Tooley PW, Fry WE (1985) Field assessment of fitness of isolates of Phytophthora infestans. Phytopathology 75:982–988
Acknowledgments
The authors thank Dr. W. E. Fry of Cornell University, Ithaca, NY, for providing the RG57 probe.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Gotoh, K., Akino, S., Kato, M. et al. New multilocus genotypes of Phytophthora infestans in Japan. J Gen Plant Pathol 73, 349–352 (2007). https://doi.org/10.1007/s10327-007-0027-7
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10327-007-0027-7