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Adsorption of OP1-related bacteriophages requires the gene encoding a TonB-dependent receptor-like protein in Xanthomonas oryzae pv. oryzae

  • Bacterial and Phytoplasma Diseases
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Abstract

Xanthomonas oryzae pv. oryzae strain T7174R is lysed by bacteriophage OP1h and OP1h2. Three mutants tolerant to both OP1h and OP1h2 were isolated by transposon mutagenesis. The mutants had an insertion of the transposon in XOO1687, which is predicted to encode a TonB-dependent receptor gene. Plasmid pHMIroNB that contained XOO1687 of T7174R was constructed, and the mutant was transformed with the plasmid. The transformant recovered sensitivity to OP1h and OP1h2. Electron microscopic analysis demonstrated that OP1h and OP1h2 can adsorb to the wild type and the transformant, but they could not adsorb to the phage-tolerant mutant. These results suggest that the TonB-dependent receptor gene relates to adsorption and infection of T7174R by OP1h2 and OP1h.

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Authors and Affiliations

  1. National Agricultural Research Center, Tsukuba, Ibaraki, 305-8666, Japan

    Yasuhiro Inoue, Jun Ohnishi, Takayuki Matsuura & Koji Azegami

  2. Graduate School of Agriculture, Kyoto Prefectural University, Kyoto, Japan

    Seiji Tsuge

  3. National Institute of Agrobiological Sciences, Tsukuba, Japan

    Hirokazu Ochiai & Hisatoshi Kaku

Authors
  1. Yasuhiro Inoue
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  2. Seiji Tsuge
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  3. Jun Ohnishi
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  4. Takayuki Matsuura
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  5. Hirokazu Ochiai
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  6. Hisatoshi Kaku
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  7. Koji Azegami
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Correspondence to Yasuhiro Inoue.

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Y. Inoue and S. Tsuge have contributed equally to this work.

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Inoue, Y., Tsuge, S., Ohnishi, J. et al. Adsorption of OP1-related bacteriophages requires the gene encoding a TonB-dependent receptor-like protein in Xanthomonas oryzae pv. oryzae . J Gen Plant Pathol 73, 365–369 (2007). https://doi.org/10.1007/s10327-007-0023-y

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  • DOI: https://doi.org/10.1007/s10327-007-0023-y

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