Abstract
In DAS-ELISAs of 86 grapevine samples from northwestern Iran, Grapevine fanleaf virus (GFLV) was detected in 18 samples. RT-PCR with two primer pairs (M2/M4 or M0/M4) corresponding to GFLV movement protein (MP) amplified the expected 854- and/or 1,489-bp fragment(s), respectively, from all ELISA-positive samples. Four smaller and three larger PCR products were cloned and sequenced, which revealed that the MP region of the isolates was 1,044 nucleotides (nt) long, corresponding to the GFLV MP. There were 83–86% nucleotide and 93–94% amino acid identities deduced between the MPs of the sequenced isolates. Nucleotide sequence identities of 81–87 and 75–79% were found between the MP regions of these isolates and that of previously published GFLV and Arabis mosaic virus (ArMV) strains/isolates, respectively. On a consensus parsimony tree based on the nucleotide sequences, isolates La208 and X300 remained distinct from previously reported GFLVs. This is the first molecular characterization of GFLV MP isolates from Iran.
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Acknowledgments
Thanks to Vice Chancellor for Research Affairs of University of Tabriz for funding, to Dr Thierry Wetzel for the primers, to Mr Ismael Torabi for technical assistance and to Dr Sassan Asgari for reviewing this manuscript.
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The sequence data reported in this paper have been submitted to the DDBJ/EMBL/GenBank databases and have been assigned accession numbers DQ286901 to DQ286916.
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Bashir, N.S., Nikkhah, S. & Hajizadeh, M. Distinct phylogenetic positions of Grapevine fanleaf virus isolates from Iran based on the movement protein gene. J Gen Plant Pathol 73, 209–215 (2007). https://doi.org/10.1007/s10327-007-0012-1
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DOI: https://doi.org/10.1007/s10327-007-0012-1