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The genetic structure of populations of Turnip mosaic virus in Kyushu and central Honshu, Japan

  • Viral and Viroid Diseases
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Abstract

The genetic structure of the populations of Turnip mosaic virus in Kyushu and central Honshu, Japan was assessed. The host specificity of isolates was determined, and their gene sequences compared utilizing a population genetic approach. Phylogenetic analysis of partial sequences revealed that 32 of 49 Honshu isolates (65%) collected during 1997–2001 belonged to the basal-BR group as did 23 of 64 isolates from Kyushu. All these basal-BR isolates infected both Brassica and Raphanus plants. However, analyses of the positions of recombination sites in five regions of the genome (one third of the full sequence) showed that at least four intra-lineage recombinants were present in these populations. These analyses showed that Kyushu and Honshu shared none of these subpopulations, and genetically distinct basal-BR populations were present in the two districts. We conclude that different basal-BR subpopulations had expanded into those districts.

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Acknowledgments

We thank Eri Muraguchi, Yui Nakamizu, Akemi Sato and Kenta Tomimura (Saga University, Japan) for their careful technical assistance, and Teruo Sano (Hirosaki University, Japan) for supplying TuMV isolates. We thank Adrian Gibbs for very kindly reading the manuscript. This work was supported by Grant-in-Aid for Scientific Research no. 17580040 from the Japan Society for the Promotion of Science.

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Authors and Affiliations

  1. Laboratory of Plant Virology, Faculty of Agriculture, Saga University, 1-banchi, Honjo-machi, Saga, 840-8502, Japan

    Yasuhiro Tomitaka & Kazusato Ohshima

  2. Nagano Agricultural Experiment Station, 492, Ogawara, Suzaka, 382-0072, Nagano, Japan

    Tohru Yamashita

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  1. Yasuhiro Tomitaka
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  2. Tohru Yamashita
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  3. Kazusato Ohshima
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Corresponding author

Correspondence to Kazusato Ohshima.

Additional information

The nucleotide sequences are deposited in the DDBJ/EMBL/GenBank databases under accession numbers AB267281-AB267376.

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Tomitaka, Y., Yamashita, T. & Ohshima, K. The genetic structure of populations of Turnip mosaic virus in Kyushu and central Honshu, Japan. J Gen Plant Pathol 73, 197–208 (2007). https://doi.org/10.1007/s10327-007-0003-2

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  • DOI: https://doi.org/10.1007/s10327-007-0003-2

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