Abstract
To study the behavior and mutation of Ralstonia solanacearum in Solanum toxicarium, which is resistant to bacterial wilt, S. toxicarium was grown in aseptic culture and inoculated with R. solanacearum. Although 60%–80% of the inoculated plants were wilting after 2 to 3 days, most wilted plants had recovered by 20 days after inoculation. The pathogen was reisolated from over 98% of inoculated plant stems, but the percentage of recovery decreased the closer the isolation sites were toward the upper stem sections. Three colony types, characterized as fluidal white, nonfluidal red, and a mixture of fluidal white and nonfluidal red, were reisolated from the stems. Nonfluidal red colonies were less virulent on tomato plants than fluidal white colonies.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
K Arai Y Sakaki Y Fukunaga Y Kuroda N Matsuzoe H Iwai (1999) ArticleTitleMovement of Ralstonia solanacearum within Solanum toxicarium resistant to bacterial wilt (in Japanese with English summary) Ann Phytopathol Soc Jpn 65 315–317
S Brumbley T Denny (1990) ArticleTitleCloning of wild-type Pseudomonas solanacearum phcA, a gene that when mutated alters expression of multiple traits that contribute to virulence J Bacteriol 172 5677–5685 Occurrence Handle2211505 Occurrence Handle1:CAS:528:DyaK3cXmtVGks78%3D
S Brumbley B Carney T Denny (1993) ArticleTitlePhenotype conversion in Pseudomonas solanacearum due to spontaneous inactivation of phcA, a putative LysR transcriptional regulator J Bacteriol 175 5477–5487 Occurrence Handle8366033 Occurrence Handle1:CAS:528:DyaK2cXktFei
IW Buddenhagen A Kelman (1964) ArticleTitleBiological and physiological aspects of bacterial wilt caused by Pseudomonas solanacearum Annu Rev Plant Pathol 2 203–230
H Date H Nasu M Hatamoto (1994) ArticleTitleBreakdown of resistance of eggplant rootstock (Solanum torvum Swartz) to bacterial wilt by high ambient temperature Ann Phytopathol Soc Jpn 60 483–486
TP Denny SR Baek (1991) ArticleTitleGenetic evidence that extracellular polysaccharide is a virulence factor of Pseudomonas solanacearum Mol Plant Microbe Interact 3 293–300
TP Denny FW Makini SM Brumbley (1988) ArticleTitleCharacterization of Pseudomonas solanacearum Tn5 mutants deficient extracellular polysaccharide Mol Plant Microbe Interact 1 215–223
V Grimault P Prior (1994) ArticleTitleInvasiveness of Pseudomonas solanacearum in tomato, eggplant and pepper: a comparative study Eur J Plant Pathol 100 259–267 Occurrence Handle10.1007/BF01876240
H Hara K Ono (1983) ArticleTitleEcological studies on the bacterial wilt of tobacco, caused by Pseudomonas solanacearum E.F. Smith. I. A selective medium for isolation and detection of Pseudomonas solanacearum (in Japanese with English summary) Bull Okayama Tob Exp Sta 42 127–138
AC Hayward (1991) ArticleTitleBiology and epidemiology of bacterial wilt caused by Pseudomonas solanacearum Annu Rev Phytopathol 29 65–87 Occurrence Handle10.1146/annurev.py.29.090191.000433 Occurrence Handle1:STN:280:DC%2BD1czhslamsQ%3D%3D Occurrence Handle18479193
A Husain A Kelman (1958) ArticleTitleRelation of slime production to mechanisms of wilting and pathogenicity of Pseudomonas solanacearum Phytopathology 48 155–156
M Iduhara S Takaki K Yokoyama T Marumoto (2002) ArticleTitleInduction of strains of Ralstonia solanacearum that do not produce extracellular polysaccharides (EPS) in soil and effect on disease suppressiveness (in Japanese with English summary) Soil Microorg 56 11–22
A Kelman (1954) ArticleTitleThe relationship of pathogenicity in Pseudononas solanacearum to colony appearance on tetrazolium medium Phytopathology 44 693–695
A Kelman J Hruschka (1973) ArticleTitleThe role of motility and aerotaxis in the selective increase of avirulent bacteria in still broth culture of Pseudomonas solanacearum J Gen Microbiol 76 177–188 Occurrence Handle4723067 Occurrence Handle1:STN:280:DyaE3s3jtVGisQ%3D%3D
N Matsuzoe H Okubo K Fujieda (1993) ArticleTitleResistance of tomato plants grafted on Solanum rootstocks to bacterial wilt and root-knot nematode (in Japanese with English summary) J Jpn Soc Hort Sci 61 865–872 Occurrence Handle10.2503/jjshs.61.865
H Mochizuki K Yamakawa (1979a) ArticleTitleResistance of selected eggplant cultivars and related wild Solanum species to bacterial wilt (Pseudomonas solanacearum) (in Japanese with English summary) Bull Veg Ornam Crops Res Sta 6 1–10
H Mochizuki K Yamakawa (1979b) ArticleTitlePotential utilization of bacterial-wilt resistant Solanum species as rootstock for commercial eggplant production (in Japanese with English summary) Bull Veg Ornam Crops Res Sta 6 11–18
T Murashige F Skoog (1962) ArticleTitleA revised medium for rapid growth and bioassays with tobacco tissue cultures Physiol Plant 15 473–497 Occurrence Handle10.1111/j.1399-3054.1962.tb08052.x Occurrence Handle1:CAS:528:DyaF3sXksFKm
K Nakaho (1997a) ArticleTitleDistribution and multiplication of Ralstonia solanacearum (Synonym Pseudomonas solanacearum) in tomato plants of resistant rootstock cultivar LS-89 and susceptible Ponderosa Ann Phytopathol Soc Jpn 63 83–88
K Nakaho (1997b) ArticleTitleDistribution and multiplication of Ralstonia solanacearum in stem-inoculated tomato rootstock cultivar LS-89 resistant to bacterial wilt Ann Phytopathol Soc Jpn 63 341–344
K Ozaki T Kimura (1992) ArticleTitleGrouping of Pseudomonas solanacearum on the basis of pathogenicity to Solanum plants (in Japanese with English summary) Bull Chugoku Natl Agric Exp Sta 10 49–58
S Poussier P Thoquet D Trigalet-Demery S Barthet D Meyer M Arlat A Trigaler (2003) ArticleTitleHost plant-dependent phenotypic reversion of Ralstonia solanacearum from non-pathogenic to pathogenic forms via alterations in the phcA genc Mol Microbiol 49 991–1003 Occurrence Handle12890023 Occurrence Handle10.1046/j.1365-2958.2003.03605.x Occurrence Handle1:CAS:528:DC%2BD3sXmslKltLk%3D
E Saile JA McGarvey M Schell TP Denny (1997) ArticleTitleRole of extracellular polysaccharide and endoglucanase in root invasion and colonization of tomato plants by Ralstonia solanacearum Phytopathology 87 1264–1271 Occurrence Handle1:CAS:528:DyaK2sXotVSgu7s%3D Occurrence Handle18945028
SE Seal LA Jackson JPW Young MJ Daniels (1993) ArticleTitleDifferentiation of Pseudomonas solanacearum, Pseudomonas syzygii, Pseudomonas pickettii and the blood disease bacterium by partial 16S rDNA sequencing: construction of oligonucleotide primers for sensitive detection by polymerase chain reaction J Gen Microbiol 139 1587–1594 Occurrence Handle8371118 Occurrence Handle1:CAS:528:DyaK3sXmsVGgtL0%3D
J Vasse P Frey A Trigalet (1995) ArticleTitleMicroscopic studies of intercellular infection and protoxylem invasion of tomato roots by Pseudomonas solanacearum Mol Plant Microbe Interact 8 241–251 Occurrence Handle1:CAS:528:DyaK2MXls1Okurs%3D
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Mori, T., Ozaki, K., Matsusaki, H. et al. Behavior and mutation of Ralstonia solanacearum in Solanum toxicarium grown in aseptic culture. J Gen Plant Pathol 73, 142–146 (2007). https://doi.org/10.1007/s10327-006-0332-6
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10327-006-0332-6