Journal of General Plant Pathology

, Volume 72, Issue 4, pp 247–252 | Cite as

Role of organic acids in the mechanisms of biological soil disinfestation (BSD)

  • Noriaki Momma
  • Kazuhiro Yamamoto
  • Peter Simandi
  • Masahiro Shishido


Biological soil disinfestation (BSD), or reductive soil disinfestation, achieved by amendment with organic materials such as wheat bran followed by flooding and covering the soil surface, has been used to control some soilborne diseases including Fusarium wilt and bacterial wilt of tomato. During a BSD treatment, accumulation of acetic acid and/or butyric acid was detected with high-performance liquid chromatography. Survival of Fusarium oxysporum f. sp. lycopersici or Ralstonia solanacearum was suppressed by these organic acids. Amendment of these organic acids into soil suppressed the survival of R. solanacearum at lower concentrations than the maximum detected in BSD treatment, indicating that production of these organic acids is one of the mechanisms of control. However, F. oxysporum f. sp. lycopersici in soil survived with the maximum concentrations of these organic acids achieved by BSD; thus, involvement of factors other than organic acids may be involved.

Key words

Fusarium oxysporum f. sp. lycopersici Ralstonia solanacearum Biological soil disinfestation (BSD) Acetic acid Butyric acid Tomato 


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  1. Blok, WJ, Lamers, JG, Termorshuizen, AJ, Bollen, GJ 2000Control of soilborne plant pathogens by incorporating fresh organic amendments followed by tarpingPhytopathology90253259Google Scholar
  2. Bohn, HL, McNeal, BL, O'Conner, GA 1985Soil chemistry2nd edn.WileyNew YorkGoogle Scholar
  3. Cook, RJ, Baker, KF 1983The nature and practice of biological control of plant pathogensAmerican Phytopathological SocietySt. Paul, MN, USAGoogle Scholar
  4. Delaquis, PJ, Sholberg, PL, Stanich, K 1999Disinfection of mung bean seed with gaseous acetic acidJ Food Prot62953957PubMedGoogle Scholar
  5. Gamliel, A, Stapleton, JJ 1993Characterization of antifungal compounds evolved from solarized soil amended with cabbage residuesPhytopathology83899905Google Scholar
  6. Handelsman, J, Stabb, EV 1996Biocontrol of soilborne plant pathogensPlant Cell818551869PubMedCrossRefGoogle Scholar
  7. Kubo, C, Ushio, S, Katase, M, Takeuchi, T 2005Analysis of factors involved in sterilization effect by soil reduction (abstract in Japanese)Jpn J Phytopathol71281282Google Scholar
  8. Lawongsa, P, Inubushi, K, Wada, H 1987Determination of organic acids in soil by high performance liquid chromatographySoil Sci Plant Nutr33299302Google Scholar
  9. Momma, N, Usami, T, Amemiya, A, Shishido, M 2005Factors involved in the suppression of Fusarium oxysporum f. sp. lycopersici by soil reductionSoil Microorg592733Google Scholar
  10. Okazaki, H, Nose, K 1986Acetic acid and n-butyric acid as causal agents of fungicidal activity of glucose-amended flooded soil (in Japanese)Jpn J Phytopathol52384393Google Scholar
  11. Raaijmakers, JM, Weller, DM 1998Natural plant protection by 2,4-diacetylphloroglucinol-producing Pseudomonas spp. in take-all decline soilsMol Plant Microbe Interact11144152Google Scholar
  12. Shinmura, A 2004Principle and effect of soil sterilization method by reducing redox potential of soil (in Japanese)The Phytopathological Society of Japan (PSJ) Soilborne Disease Workshop Report22212Google Scholar
  13. Shinmura, A, Sakamoto, N, Abe, H 1999Control of Fusarium root rot of Welsh onion by soil reduction (abstract in Japanese)Jpn J Phytopathol65352353Google Scholar
  14. Subbarao, KV 2002Methyl bromide alternatives – meeting the deadlinePhytopathology9213341343Google Scholar
  15. Veihmeyer, FJ, Hendrickson, AH 1949Methods of measuring field capacity and wilting percentages of soilsSoil Sci687594Google Scholar
  16. Wakimoto, S, Matsuyama, N, Takanami, Y, Tsuno, K 1993Laboratory guide for plant pathology and microbiology (in Japanese)Soft ScienceTokyoGoogle Scholar
  17. Wollum, AG 1982Cultural methods for soil microorganismsPage, ALMiller, RHKeeney, DR eds. Methods of soil analysis, part 2, chemical and microbiological properties (2nd edn)American Society of Agronomy, MadisonWI, USA781802Google Scholar

Copyright information

© The Phytopathological Society of Japan and Springer-Verlag Tokyo 2006

Authors and Affiliations

  • Noriaki Momma
    • 1
  • Kazuhiro Yamamoto
    • 2
  • Peter Simandi
    • 3
  • Masahiro Shishido
    • 1
  1. 1.Faculty of HorticultureChiba UniversityChibaJapan
  2. 2.Technology Development GroupKeiyo Gas CorporationIchikawaJapan
  3. 3.Faculty of Agriculture, Water and Environmental ManagementTessedik Samuel CollegeSzarvasHungary

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