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Localization and responsiveness of a cowpea apyrase VsNTPase1 to phytopathogenic microorganisms

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Abstract

Apyrases (NTPases) are associated with both compatible and incompatible interactions between plants and microorganisms. Previously we reported that the ATPase activities of cell-wall-bound apyrases of several leguminous plants, such as pea, cowpea, soybean, and kidney bean, were enhanced by a glycoprotein elicitor and were inhibited in a species-specific manner by mucin-type glycopeptide suppressors secreted from a pea pathogenic fungus, Mycosphaerella pinodes. In this study, we isolated two apyrase genes, VsNTPase1 and VsNTPase2, from a cDNA library of Vigna sinensis Endl. cv. Sanjakusasage. Based on phylogenetic analysis, VsNTPase1 may belong to a group that responds to environmental stimuli. In a transient assay using DNA bombardment, a fusion protein of green fluorescent protein (GFP) and the N-terminal putative signal sequence of VsNTPase1 was distributed in the nucleus, cytoplasm (cytoskeletal structure), and cell wall. On the other hand, a fusion protein of GFP and the N-terminal putative VsNTPase2-signal sequence was localized in the cytoplasm, especially in small particles (perhaps mitochondria). A recombinant VsNTPase1 expressed in Spodoptera frugiperda 21 cells responded directly to signal molecules from several phytopathogenic microorganisms. Here, we discuss the role of apyrases in recognizing and responding to exogenous signals.

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Authors and Affiliations

  1. Laboratory of Plant Pathology and Genetic Engineering, Faculty of Agriculture, Okayama University, Okayama, 700-8530, Japan

    Hirotaka Takahashi, Kazuhiro Toyoda, Yoshishige Inagaki, Yuki Ichinose & Tomonori Shiraishi

  2. Nishikawa Rubber Co. Ltd., Hiroshima, Japan

    Yuzo Hirakawa

  3. Wako Pure Chemical Industries, Ltd., Hiroshima, Japan

    Kunihiko Morishita

  4. Advanced Materials and Technology Research Laboratory, Nippon Steel Co. Ltd., Chiba, Japan

    Toshiaki Kato

Authors
  1. Hirotaka Takahashi
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  2. Kazuhiro Toyoda
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  3. Yuzo Hirakawa
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  4. Kunihiko Morishita
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  5. Toshiaki Kato
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  6. Yoshishige Inagaki
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  7. Yuki Ichinose
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  8. Tomonori Shiraishi
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Corresponding author

Correspondence to Tomonori Shiraishi.

Additional information

The nucleotide sequences of VsNTPase1 and VsNTPase2 in this article have been submitted to DDBJ as accession numbers AB196769 and AB196770, respectively.

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Takahashi, H., Toyoda, K., Hirakawa, Y. et al. Localization and responsiveness of a cowpea apyrase VsNTPase1 to phytopathogenic microorganisms. J Gen Plant Pathol 72, 143–151 (2006). https://doi.org/10.1007/s10327-005-0267-3

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  • DOI: https://doi.org/10.1007/s10327-005-0267-3

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