Abstract
Sequences of the internal transcribed spacer (ITS) region 1 were used to examine the phylogenetic relationships among races of 19 isolates of Phytophthora vignae f. sp. adzukicola and between this forma specialis and three isolates of the closely related P. vignae f. sp. vignae. The ITS 1 sequences were highly conserved (> 98.7% similarity) among representatives of both formae speciales groups. The results of this study indicate that P. vignae is a monophyletic group.
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References
RG Bhat AF Schmitthenner (1993) ArticleTitleGenetic crosses between physiologic races of Phytophthora sojae Exp Mycol 17 122–129
RG Bhat AF Olah AF Schmitthenner (1992) ArticleTitleCharacterization of universally avirulent strains of Phytophthora sojae Can J Bot 70 1175–1185
CM Brasier DEL Cooke JM Duncan (1999) ArticleTitleOrigin of a new Phytophthora pathogen through interspecific hybridization Proc Natl Acad Sci USA 96 5878–5883 Occurrence Handle10.1073/pnas.96.10.5878 Occurrence Handle10318978 Occurrence Handle1:CAS:528:DyaK1MXjtFCmsr8%3D
AR Crawford BJ Bassam A Drench DJ Maclean JAG Irwin (1996a) ArticleTitleEvolutionary relationships among Phytophthora species deduced from rDNA sequence analysis Mycol Res 100 437–443 Occurrence Handle1:CAS:528:DyaK28XjsFWnt7w%3D
AR Crawford BJ Bassam A Drench DJ Maclean JAG Irwin (1996b) ArticleTitleCorrection: evolutionary relationships among Phytophthora species deduced from rDNA sequence analysis Mycol Res 100 1218
WGD Fernando RG Linderman (1993) ArticleTitleOccurrence, distribution, and pathogenicity of the cowpea root and stem rot pathogen, Phytophthora vignae in soils of Sri Lanka Plant Dis 77 1158–1164
H Förster BM Tyler MD Coffey (1994) ArticleTitlePhytophthora sojae races have arisen by clonal evolution and by rare outcrosses Mol Plant Microbe Interact 7 780–791
H Förster MP Cummings MD Coffey (2000) ArticleTitlePhylogenetic relationships of Phytophthora species based on ribosomal ITS I DNA sequence analysis with emphasis on Waterhouse groups V and VI Mycol Res 104 1055–1061
EH Han CH Lee CS Sin EK Lee (1982) ArticleTitleAn investigation on the stem rot of red bean caused by Phytophthora vignae Purss Res Per Off Rural Dev Soil Fert Crop Prot Mycol Farm Prod Util 24 69–71
BJ Howlett AG Brownlee DI Guest GJ Adcock GI McFadden (1992) ArticleTitleThe 5S ribosomal-RNA gene is linked to large and small subunit ribosomal-RNA genes in the oomycetes, Phytophthora vignae, P. cinnamomi, P. megasperma f. sp. glycinea and Saprolegnia ferax Curr Genet 22 455–461 Occurrence Handle10.1007/BF00326410 Occurrence Handle1473177 Occurrence Handle1:CAS:528:DyaK3sXjtlyhuw%3D%3D
JK Hwang PK Qi (1984) ArticleTitleA study on Phytophthora of yardlong bean Acta Phytopathol Sin 14 193–199
CW Kao LS Leu (1982) ArticleTitlePhytophthora stem rot of cowpea caused by Phytophthora vignae Purss in Taiwan Plant Prot Bull 24 189–191
K Kitazawa S Tsuchiya F Kodama W Kamjaipai A Ogoshi K Yanagita (1978) ArticleTitlePhytophthora stem rot of adzuki bean (Phaseolus angularis) caused by Phytophthora vignae Purss Ann Phytopathol Soc Jpn 44 528–531
K Kitazawa T Suzui K Yanagita (1979) ArticleTitlePathogenicity of Phytophthora vignae Purss to adzuki bean and cowpea Ann Phytopathol Soc Jpn 45 406–408
N Kondo A Notsu S Naito S Fujita H Shimada (2004) ArticleTitleDistribution of Phytophthora vignae f. sp. adzukicola races in adzuki bean fields in Hokkaido, Japan Plant Dis 88 875–877
ECY Liew DJ MacLean JM Manners D Dawson JAG Irwin (1991) ArticleTitleUse of restriction fragment length polymorphisms to study genetic relationships between Australian and Japanese isolates of Phytophthora vignae Aust J Bot 39 335–346 Occurrence Handle1:CAS:528:DyaK38XhsVOqsLo%3D
H Makino S Fujita K Murata N Kondo A Ogoshi (1997) ArticleTitleCharacteristics of Phytophthora vignae isolates collected in Hokkaido Ann Phytopathol Soc Jpn 63 530
KJ May A Drenth JAG Irwin (2003) ArticleTitleInterspecific hybrids between the homothallic Phytophthora sojae and Phytophthora vignae Aust Plant Pathol 32 353–359 Occurrence Handle1:CAS:528:DC%2BD3sXntV2msrY%3D
RS Nirwan J Upadhyaya (1972) ArticleTitlePhytophthora blight of cowpea new to India Indian Phytopathol 25 162–163
A Notsu N Kondo S Fujita K Murata S Naito (2003) ArticleTitleNew race of Phytophthora vignae f. sp. adzukicola, the causal agent of Phytophthora stem rot of the adzuki bean J Gen Plant Pathol 69 39–41 Occurrence Handle10.1007/s10327-002-0007-x
GS Purss (1957) ArticleTitleStem rot: a disease of cowpeas caused by an undescribed species of Phytophthora Queensland J Agric Sci 14 125–154
B Sivakadadcham WGD Fernando (1991) ArticleTitleAn investigation on the stem rot of red bean caused by Phytophthora vignae Purss Res Per Off Rural Dev Soil Fert Crop Prot Mycol Farm Prod Util 24 69–71
Swofford DL (2002) PAUP*: Phylogenetic analysis using parsimony (and other methods) 4.0 beta. Sinauer Associates, Sunderland, MA, USA
JD Thompson TJ Gibson F Plewniak F Jeanmougin DG Higgins (1997) ArticleTitleThe CLUSTL-X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools Nucleic Acids Res 25 4876–4882 Occurrence Handle10.1093/nar/25.24.4876 Occurrence Handle9396791 Occurrence Handle1:CAS:528:DyaK1cXntFyntQ%3D%3D
S Tsuchiya M Yanagawa A Ogoshi (1986) ArticleTitleFormae speciales differentiation of Phytophthora vignae isolates from cowpea and adzuki bean Ann Phytopathol Soc Jpn 52 577–584
SC Whisson BJ Howlett ECY Liew DJ MacLean JM Manners JAG Irwin (1993) ArticleTitleAn assessment of genetic relationships between members of the Phytophthora megasperma complex and Phytophthora vignae using molecular markers Aust Sys Bot 6 295–308
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The nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank databases under the accession nos. AB120062–AB120080 and AB120122
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Kondo, N., Notsu, A., Fujita, S. et al. DNA sequence analysis of ribosomal ITS region 1 of Phytophthora vignae f. sp. adzukicola, the pathogen that causes stem rot of adzuki bean. J Gen Plant Pathol 71, 414–417 (2005). https://doi.org/10.1007/s10327-005-0232-1
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DOI: https://doi.org/10.1007/s10327-005-0232-1