Lavender essential oil decreases stress response of horses

Abstract

Simple and soft means are needed to manage horse stress. For instance, essential oils such as lavender oil contain active principles that may mitigate stress responses in humans and animals. Here we developed a protocol to test the effects of essential oil on equine stress response. We tested the effect of lavender oil on horse stress. Twenty-eight Welsh horse fillies were divided into two groups: horses treated with a roll-on with 2 mL of vegetable oil (control) and horses treated with 2 mL of 10% lavender essential oil in vegetable oil. Horses were then subjected to a series of stress tests. Heart rates and stress behavioral indicators were monitored during the 30 min of the stress tests. Saliva was collected. Blood from six horses was sampled after lavender essential oil application to follow plasma linalool content and antioxidant efficiency. Results show that stress indicators such as heart rate, alert postures and defecations are lower in lavender oil-treated horses. Lavender essential oil also modified salivary cortisol. Pharmacokinetics of linalool in plasma displayed a peak 20 min after lavender essential oil application, thus confirming the effect of lavender oil. Overall, our findings demonstrate that lavender essential oil reduces the response to stress of horses placed in stressful situations.

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References

  1. Amorati R, Foti MC, Valgimigli L (2013) Antioxidant activity of essential oils. J Agric Food Chem 61:10835–10847. https://doi.org/10.1021/jf403496k

    Article  CAS  Google Scholar 

  2. Duarte A, Luis A, Oleastro M, Domingues FC (2016) Antioxidant properties of coriander essential oil and linalool and their potential to control Campylobacter spp. Food Control 61:115–122. https://doi.org/10.1016/j.foodcont.2015.09.033

    Article  CAS  Google Scholar 

  3. Ferguson CE, Kleinman HF, Browning J (2013) Effect of lavender aromatherapy on acute-stressed horses. J Equine Vet Sci 33:67–69. https://doi.org/10.1016/j.jevs.2012.04.014

    Article  Google Scholar 

  4. Jager W, Buchbauer G, Jirovetz L, Fritzer M (1992) Percutaneous absorpion of lavender oil from a massage oil. J Soc Cosmet Chem 43:49–54

    Google Scholar 

  5. Hawken PAR, Fiol C, Blache D (2011) Behavioral reactivity to psychosocial stress determines the effects of lavender oil on anxiety in sheep. J Anim Sci 89(Suppl):10. https://doi.org/10.1016/j.physbeh.2011.12.005

    CAS  Article  Google Scholar 

  6. Hoferl M, Krist S, Buchbauer G (2006) Chirality influences the effects of linalool on physiological parameters of stress. Planta Med 72:1188–1192. https://doi.org/10.1055/s-2006-947202

    Article  CAS  Google Scholar 

  7. Lansade L, Simon F (2010) Horses’ learning performances are under the influence of several temperamental dimensions. Appl Anim Behav Sci 125:30–37. https://doi.org/10.1016/j.applanim.2010.02.010

    Article  Google Scholar 

  8. Lansade L, Pichard G, Leconte M (2008) Sensory sensitivities: components of a horse’s temperament dimension. Appl Anim Behav Sci 114:534–553. https://doi.org/10.1016/j.applanim.2008.02.012

    Article  Google Scholar 

  9. Lansade L, Valenchon M, Foury A, Neveux C, Cole SW, Laye S, Cardinaud B, Levy F, Moisan M-P (2014) Behavioral and transcriptomic fingerprints of an enriched environment in horses (Equus caballus). PLoS ONE 9:1–19. https://doi.org/10.1371/journal.pone.0114384

    Article  CAS  Google Scholar 

  10. Letizia CS, Cocchiara J, Lalko J, Api AM (2003) Fragrance material review on linalyl acetate. Food Chem Tox 41:965–976. https://doi.org/10.1016/s0278-6915(03)00014-0

    Article  CAS  Google Scholar 

  11. Muir WW (2013) Stress and pain: their relationship to health related quality of life (HRQL) for horses. Equine Vet J 45:655–656. https://doi.org/10.1111/evj.12152

    Article  Google Scholar 

  12. Seol GH, Kang P, Lee HS (2016) Antioxidant activity of linalool in patients with carpal tunnel syndrome. BMC Neurol 16:1471–2377. https://doi.org/10.1186/s12883-016-0541-3

    Article  CAS  Google Scholar 

  13. Schuwald AM, Nöldner M, Wilmes T, Klugbauer N, Leuner K, Müller WE (2013) Lavender oil-potent anxiolytic properties via modulating voltage dependent calcium channels. PLoS One 8:e59998. https://doi.org/10.1371/journal.pone.0059998

    Article  CAS  Google Scholar 

  14. Shaw D, Annett JM, Doherty B, Leslle JC (2007) Anxiolytic effects of lavender oil inhalation on open-field behaviour in rats. Phytomedicine 14:613–620. https://doi.org/10.1016/j.phymed.2007.03.007

    Article  CAS  Google Scholar 

  15. Valenchon M, Levy F, Fortin M, Leterrier C, Lansade L (2013) Stress and temperament affect working memory performance for disappearing food in horses, Equus caballus. Anim Behav 86:1233–1240. https://doi.org/10.1016/j.anbehav.2013.09.026

    Article  Google Scholar 

  16. Valgimigli L, Iori R (2009) Antioxidant and pro-oxidant capacities of ITCs. Env Mol Mutag 50:222–237. https://doi.org/10.1002/em.20468

    Article  CAS  Google Scholar 

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Acknowledgements

The authors are grateful to Fabrice Reigner and Jean Marie Yvon (INRA Nouzilly, France) for their efficient help and for the blood sampling.

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Correspondence to Anne Poutaraud.

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Poutaraud, A., Guilloteau, L., Gros, C. et al. Lavender essential oil decreases stress response of horses. Environ Chem Lett 16, 539–544 (2018). https://doi.org/10.1007/s10311-017-0681-8

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Keywords

  • Horse
  • Stress
  • Lavandula angustifolia
  • Essential oils
  • Pharmacokinetic
  • Antioxidant