Environmental Chemistry Letters

, Volume 6, Issue 2, pp 71–75 | Cite as

Analysis and decomposition of condensed tannins in tree leaves

Original Paper

Abstract

The decomposition of leaf litter is controlled by several factors. One factor that may play an important role is the content of condensed tannins (proanthocyanidins). Here we designed a combined method to isolate proanthocyanidins from leaf extracts, to convert them to anthocyanidins, and to quantify individual anthocyanidins exactly with a new, simple, but sensitive high-performance liquid chromatography method. We used this method to show composition of proanthocyanidins and to monitor degradation of proanthocyanidins and individual constituents in leaf litter in an aquatic environment over time. Despite the rapid decrease in the initial concentrations, a fraction of the proanthocyanidins remained detectable for several weeks.

Keywords

Polyphenolic compounds Proanthocyanidins Anthocyanidins Leaf litter decomposition Tannins Degradation of condensed tannins 

Notes

Acknowledgments

This study is a result of WW-DECOEX, a worldwide comparison experiment on aquatic leaf litter decomposition. We thank Guillermo Rueda-Delgado (UTADEO, Colombia), Mercedes Marchese (INALI, Santa Fé, Argentina), and Catherine Yule (Monash University, Kuala Lumpur, Malaysia) for contributing leaf samples from their research sites. This study received financial support by the Deutsche Forschungs-gemeinschaft (DFG WA 1612/2-1). Publication No. 3 of the WW-DECOEX project. We dedicate this paper to the memory of the late Rainer Suetfeld.

References

  1. Hagerman AE (1998) Tannin analysis http://www.users.muohio.edu/hagermae/tannin.pdf
  2. Mila I, Scalbert A, Expert D (1996) Iron withholding by plant polyphenols and resistance to pathogens and rots. Phytochemistry 42(6):1551–1555CrossRefGoogle Scholar
  3. Mole S, Watermann PG (1987) A critical analysis of techniques for measuring tannins in ecological studies. Oecologia 72:137–147CrossRefGoogle Scholar
  4. Porter LJ, Hrstich LN, Chan BG (1986) The conversion of procyanidins and prodelphinidins to cyanidin and delphinidin. Phytochemistry 25(1):223–230CrossRefGoogle Scholar
  5. Sarni-Manchado P, Deleris A, Avallone S, Cheynier V, Moutounet M (1999) Analysis and characterization of wine condensed tannins precipitated by proteins used as fining agent in enology. Am J Enol Vitic 50(1):81–86Google Scholar
  6. Scalbert A (1991) Antimicrobial properties of tannins. Phytochemistry 30(12):3875–3883CrossRefGoogle Scholar
  7. Schofield P, Mbugua DM, Pell AN (2001) Analysis of condensed tannins: a review. Anim Feed Sci Technol 91:21–40CrossRefGoogle Scholar
  8. Valachovic YS, Caldwell BA, Cromack K, Griffiths RP (2004) Leaf litter chemistry controls on decomposition of Pacific Northwest trees and woody shrubs. Can J For Res 34:2131–2147CrossRefGoogle Scholar
  9. Wantzen KM, Wagner R, Suetfeld R, Junk WJ (2002) How do plant-herbivore interactions of trees influence coarse detritus processing by shredders in aquatic ecosystems of different latitudes? Verh Int Verein Limnol 28:815–821Google Scholar
  10. Zucker WV (1983) Tannins: does structure determine function? An ecological perspective. Am Nat 121(3):335–365CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  1. 1.Aquatic-Terrestrial Interaction GroupUniversity of KonstanzKonstanzGermany

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