Environmental Chemistry Letters

, Volume 7, Issue 1, pp 61–65 | Cite as

Fluorescence analysis of humic-like substances extracted from composts: influence of composting time and fractionation

  • C. Richard
  • G. Guyot
  • O. Trubetskaya
  • O. Trubetskoj
  • M. Grigatti
  • L. Cavani
Original Paper

Abstract

Humic-like acids (HLA0, HLA70, HLA130, HLA730) were extracted from composts obtained from sewage sludges and trimmings after 0, 70, 130 and 730 days of composting, respectively. In addition, HLA130 was fractionated using SEC-PAGE set-up. Fluorescence spectroscopy revealed that HLA0 was the only sample to contain emission bands characteristic of protein-like compounds (λexc/λem: 280/350) and chlorophyll (λexc/λem: 420/660). Emission intensities above 400 nm and HIX both varied in the order: HLA70 > HLA130 > HLA730 > HLA0 and increased as the fraction molecular size decreased. Thus, the formation of long wavelength emitting fluorophores during composting is connected to the humification process. These fluorophores are mainly concentrated in the low molecular size fraction obtained by SEC-PAGE fractionation of composts.

Keywords

Compost humic-like substances Composting time SEC-PAGE fractionation Excitation–emission matrix 

Notes

Acknowledgment

The work has been supported by INTAS grant 05-8055.

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • C. Richard
    • 1
  • G. Guyot
    • 1
  • O. Trubetskaya
    • 2
  • O. Trubetskoj
    • 3
  • M. Grigatti
    • 4
  • L. Cavani
    • 4
  1. 1.Laboratoire de Photochimie Moléculaire et Macromoléculaire, UMR No 6505CNRS, Université Blaise PascalAubière CedexFrance
  2. 2.Branch of Shemyakin and Ovchinnikov Institute of Bioorganic ChemistryRussian Academy of SciencesMoscowRussia
  3. 3.Institute of Fundamental Problems in BiologyRussian Academy of SciencesMoscowRussia
  4. 4.Dip. di Scienze e Tecnologie AgroambientaliAlma Mater Studiorum, Università di BolognaBolognaItaly

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