Humic-like acids (HLA0, HLA70, HLA130, HLA730) were extracted from composts obtained from sewage sludges and trimmings after 0, 70, 130 and 730 days of composting, respectively. In addition, HLA130 was fractionated using SEC-PAGE set-up. Fluorescence spectroscopy revealed that HLA0 was the only sample to contain emission bands characteristic of protein-like compounds (λexc/λem: 280/350) and chlorophyll (λexc/λem: 420/660). Emission intensities above 400 nm and HIX both varied in the order: HLA70 > HLA130 > HLA730 > HLA0 and increased as the fraction molecular size decreased. Thus, the formation of long wavelength emitting fluorophores during composting is connected to the humification process. These fluorophores are mainly concentrated in the low molecular size fraction obtained by SEC-PAGE fractionation of composts.
Compost humic-like substances Composting time SEC-PAGE fractionation Excitation–emission matrix
This is a preview of subscription content, log in to check access.
The work has been supported by INTAS grant 05-8055.
Alberts JJ, Takacs M (2004) Total luminescence spectra of IHSS standard and reference fulvic acids, humic acids and natural organic matter: comparison of aquatic and terrestrial source terms. Org Geochem 35:243–256CrossRefGoogle Scholar
Amine-Khodja A, Trubetskaya O, Trubetskoj O, Cavani L, Ciavatta C, Guyot G, Richard C (2006a) Humic-like substances extracted from composts can promote the photodegradation of Irgarol 1051 in solar light. Chemosphere 62:1021–1027CrossRefGoogle Scholar
Amine-Khodja A, Richard C, Lavédrine B, Guyot G, Trubetskaya O, Trubetskoj O (2006b) Water-soluble fractions of composts for the photodegradation of organic pollutants in solar light. Environ Chem Lett 3:173–177CrossRefGoogle Scholar
Chen Y, Senesi N, Schnitzer M (1977) Information provided on humic substances by E4/E6 ratios. Soil Sci Soc Am J 41:352–358CrossRefGoogle Scholar
Chen W, Westerhoff P, Leenheer JA, Booksh K (2003) Fluorescence excitation-emission matrix regional integration to quantify spectra for dissolved organic matter. Environ Sci Technol 37:5701–5710CrossRefGoogle Scholar
Coble C (1996) Characterization of marine and terrestrial DOM in seawater using excitation–emission matrix spectroscopy. Mar Chem 51:325–346CrossRefGoogle Scholar
Fuentes M, Gonzalez-Gaitano G, Garcia-Mina JM (2006) The usefulness of UV–visible and fluorescence spectroscopies to study the chemical nature of humic substances from soils and composts. Org Geochem 37:1949–1969CrossRefGoogle Scholar
Grigatti M, Ciavatta C, Gessa C (2004) Evolution of organic matter from sewage sludge and garden trimming during composting. Biores Technol 91:163–169CrossRefGoogle Scholar
Kalbitz K, Geyer W, Geyer S (1999) Spectroscopic properties of dissolved humic substances–a reflection of land use history in a fen area. Biogeochem 47:219–238Google Scholar
Milori D, Martin-Neto L, Bayer C, Mielniczuk J, Vagnato V (2002) Humification degree of soil humic acids determined by fluorescence spectroscopy. Soil Sci 167:739–749CrossRefGoogle Scholar
Peuravuori J, Koivikko R, Pihlaja K (2002) Characterization, differentiation and classification of aquatic humic matter separated with different sorbents: synchronous scanning fluorescence spectroscopy. Water Res 36:4552–4562CrossRefGoogle Scholar
Trubetskaya OE, Trubetskoj O, Ciavatta C (2001) Evaluation of the transformation of organic matter to humic substances in compost by coupling SEC-PAGE. Biores Technol 77:51–56CrossRefGoogle Scholar
Trubetskaya OE, Trubetskoj O, Guyot G, Richard C (2002) Fluorescence properties of soil humic acids and their fractions obtained by tandem SEC-PAGE. Org Geochem 33:213–220CrossRefGoogle Scholar
Zsolnay A, Baigar E, Jimenez B, Steinberg B, Saccomandi F (1999) Differentiating with fluorescence the sources of dissolved organic matter in soils subjected to drying. Chemosphere 38:45–50CrossRefGoogle Scholar