Environmental Chemistry Letters

, Volume 7, Issue 1, pp 41–44 | Cite as

Effects of metal ions on Mg2+-ATPase activity in plasma membranes isolated from the rat uterus

  • A. Horvat
  • M. Milošević
  • M. Demajo
Original Paper


In this study, we investigated the in vitro effects of metal ions on Mg2+-ATPase activity in isolated membranes from rat uterus. The effects of increasing concentrations of metal salts (CrCl2, CuSO4, HgCl2 and ZnSO4) show sigmoidal and almost complete inhibition relative to the control enzyme activity. According to the IC50, the ATPase possesses greater sensibility to \( {\text{Zn}}^{{{\text{2}} + }} > {\text{Cu}}^{{{\text{2}} + }} \cong {\text{Cr}}^{{{\text{3}} + }} \cong {\text{Hg}}^{{{\text{2}} + }} ,\) while other metal salts exhibit the following inhibition: CdCl2 55%, CsCl 64.5% and SrCl2 58%. Here we demonstrated that the physico-chemical properties of these metals are of importance in defining possible mechanisms of binding and decrease of enzyme activity.


Mg2+-ATPase Uterus Membrane Metal ions Rat 



This study was supported by the Ministry of Science of the Republic of Serbia, Project No 143044B.


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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  1. 1.Laboratory for Molecular Biology and Endocrinology“Vinča” Institute of Nuclear SciencesBelgradeSerbia

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