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Determination of selected neonicotinoid insecticides by liquid chromatography with thermal lens spectrometric detection


We studied the analysis of trace amounts of neonicotinoid insecticides by liquid chromatography coupled with a thermal lens spectrometric detector (TLS). This multi-residue analysis method is based on the reversed phase separation on C18 column, isocratic elution and collinear dual beam TLS detection. The insecticides thiamethoxam, imidacloprid, acetamiprid and thiacloprid were detected with retention times of 4.4, 5.7, 6.5 and 8.5 min and limits of quantifications of 50, 89, 10, and 25 μg/L, respectively. The retention times agreed well with those obtained by the same chromatographic method but using a diode-array detector (DAD). The limits of quantifications for imidacloprid were identical in both techniques. However, the limits of quantifications for thiamethoxam, acetamiprid and thiacloprid were up to 8.5 times lower using the TLS detector compared to the diode-array detector. The applicability of the developed procedure was tested on spiked river water and potato samples.

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The authors acknowledge financial support of the Secretariat for Science and Technological Development, Autonomous Province of Vojvodina, R. Serbia (Grant no. 114-451-00604/2005-01), the financial support of the European Community allotted by the European Agency for Reconstruction through the Ministry of International Economic Relations of the Republic of Serbia whithin the Neighbourhood Programme Hungary-Serbia (Grant no. 04SER02/01/009), the Foundation János Arany (Hungary) and of Slovenian Research Agency Grant no.: J1-6001 SLO-SCG bilateral exchange grants.

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Correspondence to Mladen Franko.

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Guzsvány, V., Madžgalj, A., Trebše, P. et al. Determination of selected neonicotinoid insecticides by liquid chromatography with thermal lens spectrometric detection. Environ Chem Lett 5, 203–208 (2007).

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  • Neonicotinoid insecticides
  • Quantitation
  • Potato
  • River water