We studied the analysis of trace amounts of neonicotinoid insecticides by liquid chromatography coupled with a thermal lens spectrometric detector (TLS). This multi-residue analysis method is based on the reversed phase separation on C18 column, isocratic elution and collinear dual beam TLS detection. The insecticides thiamethoxam, imidacloprid, acetamiprid and thiacloprid were detected with retention times of 4.4, 5.7, 6.5 and 8.5 min and limits of quantifications of 50, 89, 10, and 25 μg/L, respectively. The retention times agreed well with those obtained by the same chromatographic method but using a diode-array detector (DAD). The limits of quantifications for imidacloprid were identical in both techniques. However, the limits of quantifications for thiamethoxam, acetamiprid and thiacloprid were up to 8.5 times lower using the TLS detector compared to the diode-array detector. The applicability of the developed procedure was tested on spiked river water and potato samples.
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Di Muccio A, Fidente P, Barbini AD, Dommarco R, Seccia S, Morrica P (2006) Application of solid-phase extraction and liquid chromatography-mass spectrometry to the determination of neonicotinoid pesticide residues in fruit and vegetables. J Chromatogr A 1108:1–6
Fidente P, Seccia S, Vanni F, Morrica P (2005) Analysis of neonicotinoid insecticides residues in honey by solid matrix partition clean-up and liquid chromatography-electrospray mass spectrometry. J Chromatogr A 1094:175–178
Franko M (2001) Recent applications of thermal lens spectrometry in food analysis and environmental research. Talanta 54:1–13
Fernandez-Alba RA, Valverde A, Aügera A, Contreras M, Chiron S (1996) Determination of imidacloprid in vegetables by high-performance liquid chromatography with diode-array detection. J Chromatogr A 721:97–105
Iwasa T, Motoyama N, Ambrose JT, Michael Roe R (2004) Mechanism for the different toxicity of neonicotinoid insecticides in the honey bee, Apis mellifera. Crop Protection 23:371–378
Luterotti S, Franko M, Šikovec M, Bicanic D (2002) Ultarsensitive assays of trans- and cis-β-carotenes in vegetable oils by high-performance liquid chromatography-thermal lens detection. Anal Chim Acta 460:193–200
Mandić IA, Lazić DS, Ökrész NSz, Gaál FF (2005) Determination of the insecticide imidacloprid in potato (Solanum tuberosum L.) and onion (Allium cepa) by high-performance liquid chromatography with diode-array detection. J Anal Chem 60:1134–1138
Miller JN, Miller JC (1988) Statistics for analytical chemistry, 2nd edn. Ellis Horwood, Chischester, p 112
Obana H, Okihashi M, Akutsu K, Kitagawa Y, Hori S (2002) Determination of acetamiprid, imidacloprid and nitenpiram residues in vegetables and fruits by high-performance liquid chromatography with diode-array detection. J Agric Food Chem 50:4464–4467
Obana H, Okihashi M, Akutsu K, Kitagawa Y, Hori S (2003) Determination of neonicotinoid pesticide residues in vegetables and fruits with solid phase extraction and liquid chromatography mass spectrometry. J Agric Food Chem 51:2501–2505
Pogačnik L, Franko M (2003) Detection of organophosphate and carbamate pesticides in vegetable samples by a photothermal biosensor. Biosens Bioelectron 18:1–9
Rancan M, Sabatini G A, Achilli G, Galletti C G (2006) Determination of imidacloprid and metabolites by liqid chromatography with an electrochemical detector and post column photochemical reactor. Anal Chim Acta 555:20–24
Seccia S, Fidente P, Barbini Attard D, Morrica P (2005) Multiresidue determination of neonicotinoid insecticide residues in drinking water by liquid chromatography with electrospry ionisation mass spectrometry. Anal Chim Acta 553:21–26
Šikovec M, Franko M, Novič M, Veber M (2001) Effect of organic solvents in the on-line thermal lens spectrometric detection of chromium(III) and chromium(VI) after ion chromatographic separation. J Chromatogr A 920:119–125
Tomlin C (ed) (2000) The pesticide manual: a world compendium, 12th edn. British Crop Protection Council, Farnham, United Kingdom
Vilchez J L, El-Khattabi R, Fernández J, González-Casado, Navalón A (1996) Determination of imidacloprid in water and soil samples by gas chromatography-mass spectrometry. J Chromatogr A 746:289–294
The authors acknowledge financial support of the Secretariat for Science and Technological Development, Autonomous Province of Vojvodina, R. Serbia (Grant no. 114-451-00604/2005-01), the financial support of the European Community allotted by the European Agency for Reconstruction through the Ministry of International Economic Relations of the Republic of Serbia whithin the Neighbourhood Programme Hungary-Serbia (Grant no. 04SER02/01/009), the Foundation János Arany (Hungary) and of Slovenian Research Agency Grant no.: J1-6001 SLO-SCG bilateral exchange grants.
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Guzsvány, V., Madžgalj, A., Trebše, P. et al. Determination of selected neonicotinoid insecticides by liquid chromatography with thermal lens spectrometric detection. Environ Chem Lett 5, 203–208 (2007). https://doi.org/10.1007/s10311-007-0102-5
- Neonicotinoid insecticides
- River water