Abstract
We developed a cost-effective and sensitive spectrophotometric method for the determination of arsenic at trace level using a new reagent, leuco malachite green. Here we show that, arsenic reacts with potassium iodate in acidic conditions to liberate iodine, and the liberated iodine selectively oxidizes leuco malachite green to malachite green dye. We studied the Beer’s law at 617 nm, which showed linearity over the concentration range 0.09–0.9 μg ml−1 of arsenic. We show that the molar absorptivity, Sandell’s sensitivity and detection limit of the method are 6.1 × 104 l mol−1 cm−1, 0.0012 μg cm−2 and 0.025 μg ml−1, respectively. We applied the developed method for the determination of arsenic in environmental samples.
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References
Almond H (1953) Field method for determination of traces of arsenic in soils. Anal Chem 25:1766–1767 doi:10.1021/ac60083a056
AWWA (1992) Water quality and treatment. American water works association, Washington, DC, p 83
ICH Guidelines (2005) Validation of analytical procedures: text and methodology, Topic Q2(R1), Step 4 November, Part II, pp 11–12
Kohlmeyer U, Jantzen E, Kuballa J, Jakubik S (2003) Benefits of high resolution IC-ICP-MS for the routine analysis of inorganic and organic arsenic species in food products of marine and terrestrial origin. Anal Bioanal Chem 377:6–13 doi:10.1007/s00216-003-2064-1
Maher WA (1983) Spectrophotmetric determination of arsenic in biological tissues and sediments after digestion with nitric, sulphuric and perchloric acids and pre-concentration by zinc column arseine generation and trapping. Analyst 108:939–943
Marczenco Z (1986) Separation and spectrophotometric determination of elements, 2nd edn. Ellis Horwood Ltd, Chichester, pp 149–158
Michael AD, Rogers IB (1954) Spectrophotometric determination of arsenic, phosphorus, and silicon in presence of each other. Anal Chem 26:1381–1383 doi:10.1021/ac60092a040
National Research Council (2001) Arsenic in drinking water. National Academic, Washington DC
Patty FA (1963) Industrial hygiene and toxicology, vol II, Wiley Interscience, New York, p 871
Pillai A, Sunitha G, Gupta VK (2000) A new system for the spectrophotometric determination of arsenic in environmental and biological samples. Anal Chim Acta 408:111–115
Acknowledgments
One of the authors (B.P. Dayananda) is grateful to the University Grants Commission, New Delhi and Department of Collegiate Education, Government of Karnataka for awarding a Teacher Fellowship.
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Revanasiddappa, H.D., Dayananda, B.P. & Kumar, T.N.K. A sensitive spectrophotometric method for the determination of arsenic in environmental samples. Environ Chem Lett 5, 151–155 (2007). https://doi.org/10.1007/s10311-007-0097-y
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DOI: https://doi.org/10.1007/s10311-007-0097-y