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Development of 18 microsatellite markers in Pieris japonica, a poisonous tree insulated from the browsing pressure of herbivores, using a next-generation sequencer

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Journal of Forest Research

Abstract

Pieris japonica is a poisonous tree species that is rarely eaten by herbivorous animals, which could allow it to expand its distribution and change ecosystems. Using a next-generation sequencer, 18 microsatellite markers were isolated from P. japonica and characterized. The number of alleles at each locus ranged from 2 to 11. The observed and expected heterozygosities ranged from 0.13 to 1.00 and 0.32 to 0.88, respectively. These markers will be useful for genetic studies of P. japonica, which will be essential for conservation of its surrounding environment.

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Acknowledgments

We thank the Tokyo University Forest of Chichibu, Japan for allowing us to sample our target species. This study was supported in part by Grant in aid from the joint project between the University of Tokyo Chichibu Forest and SUNTRY to H.K.

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Authors and Affiliations

  1. Asian Natural Environmental Science Center, The University of Tokyo, 1-1-8, Midori-cho, Nishitokyo, Tokyo, 188-0002, Japan

    Hiroyuki Kurokochi & Chunlan Lian

  2. Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan

    Engkong Tan & Shuichi Asakawa

Authors
  1. Hiroyuki Kurokochi
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  2. Engkong Tan
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  3. Shuichi Asakawa
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  4. Chunlan Lian
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Correspondence to Hiroyuki Kurokochi.

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Kurokochi, H., Tan, E., Asakawa, S. et al. Development of 18 microsatellite markers in Pieris japonica, a poisonous tree insulated from the browsing pressure of herbivores, using a next-generation sequencer. J For Res 20, 244–247 (2015). https://doi.org/10.1007/s10310-014-0456-z

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  • DOI: https://doi.org/10.1007/s10310-014-0456-z

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