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Journal of Forest Research

, Volume 19, Issue 2, pp 260–267 | Cite as

Ectomycorrhizal fungal communities of Dipterocarpus alatus seedlings introduced by soil inocula from a natural forest and a plantation

  • Tharnrat Kaewgrajang
  • Uthaiwan Sangwanit
  • Motoichiro Kodama
  • Masahide Yamato
Original Article

Abstract

We attempted to introduce ectomycorrhizal (ECM) fungi onto seedlings of Dipterocarpus alatus (Dipterocarpaceae) by soil inocula collected from tree stands of D. alatus. Top soils collected from beneath the trees of D. alatus in a dry evergreen forest and a 15-year-old plantation were inoculated onto germinating seeds of D. alatus. After 7 months of seedling cultivation, ECM fungal communities in the seedlings were investigated based on the sequences of ITS rDNA. The ECM fungi detected were divided into 19 phylotypes by molecular analysis. Most of the phylotypes were identified as ECM fungal taxa, i.e., Clavulina, Laccaria, Lactarius, Tomentella, Pyronemataceae, and Tricholomataceae. Accordingly, we can confirm that soil inoculation is a simple method to induce ECM formation with diverse fungi in pot cultured seedlings, which would be useful for introducing diverse ECM fungi to dipterocarp plantations.

Keywords

Dipterocarpaceae Ectomycorrhiza ITS rDNA Tomentella Tropical forest 

Notes

Acknowledgments

We thank the Dean of the Faculty of Forestry, Kasetsart University, Bangkok, who provided the forest nursery and soil sampling sites in Wang Nam Khiew Training Camp, and the head of Sakaerat Silvicultural Research Station in the Royal Forest Department who provided the soil sampling site in the plantation. This study was supported by the Global Center of Excellence Program “Advanced utilization of fungus/mushroom resources for sustainable society in harmony with nature” from the Ministry of Education, Culture, Sports, Science, and Technology of Japan. We also thank the editor and two anonymous reviewers for their valuable comments on the manuscript.

Supplementary material

10310_2013_408_MOESM1_ESM.tif (6 mb)
Ectomycorrhizal roots of Dipterocarpus alatus induced by soil inocula collected from stands of D. alatus: a morphotype 1; b cross-section of morphotype 1; c morphotype 2; d cross-section of morphotype 2; e morphotype 3; f cross-section of morphotype 3 (M mantle, arrowhead Hartig net, white bar 400 μm, black bar 10 μm) (TIFF 6189 kb)

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Copyright information

© The Japanese Forest Society and Springer Japan 2013

Authors and Affiliations

  • Tharnrat Kaewgrajang
    • 1
  • Uthaiwan Sangwanit
    • 2
  • Motoichiro Kodama
    • 3
  • Masahide Yamato
    • 3
    • 4
  1. 1.The United Graduate School of Agricultural ScienceTottori UniversityTottoriJapan
  2. 2.Department of Forest Biology, Faculty of ForestryKasetsart UniversityBangkokThailand
  3. 3.Fungus/Mushroom Resources and Research Center, Faculty of AgricultureTottori UniversityTottoriJapan
  4. 4.Department of Science Education, Faculty of EducationChiba UniversityChibaJapan

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