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The effect of cold stress on endogenous hormones and CBF 1 homolog in four contrasting bamboo species

  • Original Article
  • Published:
Journal of Forest Research

Abstract

An unusually severe snow disaster in southern China in 2008 exposed variation among different species of bamboo: some species were highly tolerant to low-temperature damage, but some suffered extensive damage or death. Therefore, this study was conducted to determine the effects of cold stress on endogenous hormones and the expression of homologs of CBF 1 in four bamboo species differing in their tolerance to cold. All four bamboo species were subjected to cold stress with a temperature gradient (10, 5, 0, −5, and −10°C), and each low temperature treatment was maintained for 48 h. Results showed that ABA, GA1, and GA4 were significantly affected by cold stress: ABA increased with cold stress in all species, while GA1 and GA4 reduced with cold stress. ABA levels and the ratio of ABA/(GA1 + GA4) in leaves of Neosinocalamus affinis and Bambusa rigida were both higher than those of hybrid bamboo (Bambusa pervariabilis × Dendrocalamopsis grandis) and Dendrocalamus latiflorus for all treatments, while GA1 and GA4 were conversely lower as compared to the hybrid variety and D. latiflorus. Moreover, CBF 1 homolog was up-regulated during cold stress, with higher expression levels in the leaves of cold-tolerant species than cold-sensitive ones. Results revealed that high levels of ABA and CBF 1 , as well as the ABA/GAs balance, are important in increased plant resistance to cold stress.

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References

  • Arora R, Wisniewski M (1994) Cold acclimation in genetically related (sibling) deciduous and evergreen peach (Prunus persica (L.) Batsch): a 60-kilodalton bark protein in cold-acclimated tissues of peach is heat stable and related to the dehydrin family of proteins. Plant Physiol 105:95–101

    Article  PubMed  CAS  Google Scholar 

  • Arora R, Wisniewski M, Rowland LJ (1996) Cold acclimation and alterations in dehydrin-like and bark storage proteins in the leaves of sibling deciduous and evergreen peach. J Am Soc Hortic Sci 121:915–919

    CAS  Google Scholar 

  • Badr SA, Martin GC, Hartmann HT (1971) A modified method for extraction and identification of abscisic acid and gibberellin–like substances from the olive (Olea europaea). Physiol Plant 24:191–198

    Article  CAS  Google Scholar 

  • Baier M, Hartung W (1991) Cytoplasmic pH and abscisic acid transport across guard cell membranes. Z Naturforsch C 46:106–110

    CAS  Google Scholar 

  • Barnes JD, Wilson JM (1986) Effects of hormones on morphogenesis and mold resistance in berseem clover (Trifolium alexandrinum L.). J Exp Bot 37:1542–1551

    Article  CAS  Google Scholar 

  • Campalans A, Pages M, Messeguer R (2000) Protein analysis during almond embryo development: identification and characterization of a late embryogenesis abundant protein. Plant Physiol Biochem 38:449–457

    Article  CAS  Google Scholar 

  • Chen KS, Xu CJ, Li QF, Chen J, Zhang SL (2003) An improved HPLC method for analyzing endogenous IAA and ABA in fruit tissues. J Fruit Sci 20:4–7 (In Chinese)

    CAS  Google Scholar 

  • Crosatti C, Nevo E, Stanca AM, Cattivelli L (1996) Genetic analysis of the accumulation of COR14 proteins in wild (Hordeum spontaneum) and cultivated (Hordeum vulgare) barley. Theor Appl Genet 93:975–981

    Article  CAS  Google Scholar 

  • Daeter W, Hartung W (1995) Stress-dependent redistribution of abscisic acid (ABA) in Hordeum vulgare L. leaves: the role of epidermal ABA metabolism, the tonoplastic transport and the cuticle. Plant Cell Environ 18:1367–1376

    Article  CAS  Google Scholar 

  • Daie J, Campbell WF (1981) Response of tomato plants to stress temperatures: increase in abscisic acid concentrations. Plant Physiol 67:26–29

    Article  PubMed  CAS  Google Scholar 

  • Ding J, Shen YX, Fang XX, Feng L, Ni JS (1979) Abstraction, separation and bio-identification of endogenous hormones in plant. Plant Physiol Commun 2:27–29 (In Chinese)

    Google Scholar 

  • Ding YH, Wang ZY, Song YF, Zhang J (2008) The unprecedented freezing disaster in January 2008 in southern China and its possible association with the globe warming. Acta Meteorol Sin 22:538–558 (In Chinese)

    Google Scholar 

  • Else MA, Davies WJ, Whitford PN, Hall KC, Jackson MB (1994) Concentrations of abscisic acid and other solutes in xylem sap from root systems of tomato and castor oil plants are distorted by wounding and variable sap flow rates. J Exp Bot 45:317–323

    Article  CAS  Google Scholar 

  • Fowler S, Thomashow MF (2002) Arabidopsis transcriptome profiling indicates that multiple regulatory pathways are activated during cold acclimation in addition to the CBF cold response pathway. Plant Cell 14:1675–1690

    Article  PubMed  CAS  Google Scholar 

  • Gilmour SJ, Sebolt AM, Salazar MP, Everard JD, Thomashow MF (2000) Overexpression of the Arabidopsis CBF3 transcriptional activator mimics multiple biochemical changes associated with cold acclimation. Plant Physiol 24:1854–1865

    Article  Google Scholar 

  • Guy CL (1990) Cold acclimation and freezing stress tolerance: role of protein metabolism. Annu Rev Plant Physiol Plant Mol Biol 41:187–223

    Article  CAS  Google Scholar 

  • Hamayun M, Khan SA, Shinwari ZK, Khan AL, Ahmad N, Lee IJ (2010) Effect of polyethylene glycol induced drought stress on physio-hormonal attributes of soybean. Pak J Bot 42:977–986

    CAS  Google Scholar 

  • Hughes MA, Dunn MA (1996) The molecular biology of plant acclimation to low temperature. J Exp Bot 47:291–305

    Article  CAS  Google Scholar 

  • Jaglo-Ottosen KR, Gilmour SJ, Zarka DG, Schabenberger O, Thomashow MF (1998) Arabidopsis CBF1 overexpression induces COR genes and enhances freezing tolerance. Science 280:104–106

    Article  PubMed  CAS  Google Scholar 

  • Jiang JM, Li BX, Jiang NQ, Zhu WS, Yu Y, Chen XM (2008) Impact of the snow disaster occurred in 2008 in south China to the clump bamboo in south Sichuan. Sci Silvae Sin 44:141–144 (In Chinese)

    Google Scholar 

  • Joo GJ, Kim YM, Kim JT, Rhee IK, Kim JH, Lee IJ (2005) Gibberellins-producing rhizobacteria increase endogenous gibberellins content and promote growth of red peppers. J Microbiol 12:510–515

    Google Scholar 

  • Kitashiba H, Ishizaka T, Isuzugawa K, Nishimura K, Suzuki T (2004) Expression of a sweet cherry DREB1/CBF ortholog in Arabidopsis confers salt and freezing tolerance. J Plant Physiol 161:1171–1176

    Article  PubMed  CAS  Google Scholar 

  • Kuwabara C, Takezawa D, Shimada T, Hamada T, Fujikawa S, Arakawa K (2002) Abscisic acid- and cold-induced thaumatin-like protein in winter wheat has an antifungal activity against snow mould, Microdochium nivale. Physiol Plant 115:101–110

    Article  PubMed  CAS  Google Scholar 

  • Lee IJ, Foster KR, Morgan PW (1998) Photoperiod control of gibberellin levels and flowering in Sorghum. Plant Physiol 116:1003–1010

    Article  PubMed  CAS  Google Scholar 

  • Lee JT, Prasad V, Yang PT, Wu JF, Ho THD, Chang YY, Chan MT (2003) Expression of Arabidopsis CBF1 regulated by an ABA/stress inducible promoter in transgenic tomato confers stress tolerance without affecting yield. Plant Cell Environ 26:1181–1190

    Article  CAS  Google Scholar 

  • Lee SC, Huh KW, An K, An G, Kim SR (2004) Ectopic expression of a cold-inducible transcription factor, CBF1/DREB1b in transgenic rice (Oryza sativa L.). Mol Cells 18:107–114

    PubMed  CAS  Google Scholar 

  • Levitt J (1980) Responses of plants to environmental stresses. vol 1. Academic Press, New York

  • Liu Q, Kasuga M, Sakuma Y, Abe H, Miura S, Yamaguchi-Shinozaki K, Shinozaki K (1998) Two transcription factors, DREB1 and DREB2, with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought- and low-temperature-responsive gene expression, respectively, in Arabidopsis. Plant Cell 10:1391–1406

    Article  PubMed  CAS  Google Scholar 

  • Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2 −△△CT method. Methods 25:402–408

    Article  PubMed  CAS  Google Scholar 

  • Matsui T, Bhowmik PK, Yokozeki K (2004) A cDNA sequence encoding actin gene in moso bamboo shoot and its phylogenetic analysis. Asia J Plant Sci 3:128–131

    Article  Google Scholar 

  • Nakashima K, Yamaguchi-Shinozaki K (2006) Regulons involved in osmotic stress-responsive and cold stress-responsive gene expression in plants. Physiol Plant 126:62–71

    Article  CAS  Google Scholar 

  • Peng YH, Xiang WH, Tian DL, Chen FF, Zeng DQ, Zhang Y (2008) Status of frozen injury of bamboo after snow and ice disaster in Changsha. Sci Silvae Sin 44:55–58 (In Chinese)

    Google Scholar 

  • Richard S, Morency MJ, Drevet C, Jouanin L, Seguin A (2000) Isolation and characterization of a dehydrin gene from white spruce induced upon wounding, drought and cold stresses. Plant Mol Biol 43:1–10

    Article  PubMed  CAS  Google Scholar 

  • Ritchie S, Gilroy S (1998) Gibberellins: regulating genes and germination. New Phytol 140:363–383

    Article  CAS  Google Scholar 

  • Samsoedin RR, Honda I, Yanagisawa T, Kobayashi M, Kamiya Y, Takahashi N (1996) Gibberellin biosynthesis in Bambusoideae. Biosci Biotech Biochem 60:1497–1499

    Article  CAS  Google Scholar 

  • Scott TK (1979) Plant regulation and world agriculture. Plenum, New York, pp 327–371

  • Stockinger EJ, Gilmour SJ, Thomashow MF (1997) Arabidopsis thaliana CBF1 encodes an AP2 domain-containing transcriptional activator that binds to the C-repeat/DRE, a cis-acting DNA regulatory element that stimulates transcription in response to low temperature and water deficit. Proc Natl Acad Sci USA 94:1035–1040

    Article  PubMed  CAS  Google Scholar 

  • Su WH, Fan SH, Zhang WY, Qi LH, Guan FY (2008) Damage of forest and snow disaster to Moso bamboo stands and the influencing factors in Huangshan, Anhui province. Sci Silvae Sin 44:42–49 (In Chinese)

    Google Scholar 

  • Taylor IB, Burbidge A, Thompson AJ (2000) Control of abscisic acid synthesis. J Exp Bot 51:1563–1574

    Article  PubMed  CAS  Google Scholar 

  • Veisz O, Galiba G, Sutka J (1996) Effect of abscisic acid on the cold hardiness of wheat seedlings. J Plant Physiol 149:439–443

    Article  CAS  Google Scholar 

  • Verwoerd TC, Dekker BMM, Hoekema AA (1989) Small-scale procedure for the rapid isolation of plant RNAs. Nucleic Acids Res 17:2362

    Google Scholar 

  • Wilkinson S, Davies WJ (1997) Xylem sap pH increase: a drought signal received at the apoplastic face of the guard cell which involves the suppression of saturable ABA uptake by the epidermal symplast. Plant Physiol 113:559–573

    PubMed  CAS  Google Scholar 

  • Xie JR, Xiang DY, Mei H, Tan F (2002) Relationship between changes of cold resistance and endogenous hormone of Taxus chinensis var mairei Cheng et L.K.F. J Southwest China Normal Univ (Natural Science) 27:232–234 (In Chinese)

    Google Scholar 

  • Yamaguchi-Shinozaki K, Shinozaki K (2006) Transcriptional regulatory networks in cellular response and the tolerance to dehydration and cold stresses. Annu Rev Plant Biol 57:781–803

    Article  PubMed  CAS  Google Scholar 

  • Yang GY, Xu XL, Yang LS, He XB (2008) Bamboo forest damage caused by snow storm in Sichuan province in 2008 and silvicultural reestablishment measures. Sci Silvae Sin 44:96–100 (In Chinese)

    Google Scholar 

  • Yang ZH, Yan PD, Shu WB (2009) Relationships between dynamic changes of endogenous hormones and cold-resistance of improved provenance of Pinus massoniana. Guangxi Sci 1:87–91 (In Chinese)

    Google Scholar 

  • Yoon JY, Hamayun M, Lee SK, Lee IJ (2009) Methyl jasmonate alleviated salinity stress in soybean. J Crop Sci Biotechnol 12:63–68

    Article  Google Scholar 

  • Yue D, Wang YK (2008) Study on relationship between endogenous hormones and cold resistance in almond. J Anhui Agric Sci 36:9951–9952, 10023 (In Chinese)

    Google Scholar 

  • Zhang WW, Che DD, Zhang X, Wang JG, Fan JP (2005) Cloning of transcription factor CBF1 gene and construction of plant expression vector regulated separatedly by two promoters. Mol Plant Breed 3:493–497 (In Chinese)

    CAS  Google Scholar 

  • Zhao JF, Dong WY, Mao WJ, Wang L (2009) Investigation on snow damage on four kinds of bamboo forests in Daguan County. J West China For Sci 38:96–100 (In Chinese)

    Google Scholar 

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Acknowledgments

We wish to thank Dr. Brian Stanton for helpful comments on our manuscript. This work was jointly supported by the 11th Five Years Key Programs for forest breeding of Sichuan province (No. 2006YZGG), the Key Program of Sichuan Education Office (No. 08ZA072), and the Young Scientists Fund of Sichuan Agricultural University (No. 00330800; No. 00330700).

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Authors and Affiliations

  1. College of Forestry, Sichuan Agricultural University, Xinkang Road, Ya’an, 625014, Sichuan, China

    Fan Zhang & Xue Qin Wan

  2. College of Landscape Architecture, Sichuan Agricultural University, Ya’an, 625014, Sichuan, China

    Fan Zhang, Hai Qing Zhang, Guang Li Liu, Ming Yan Jiang, Yuan Zhi Pan & Qi Bing Chen

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  1. Fan Zhang
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Correspondence to Fan Zhang.

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Zhang, F., Wan, X.Q., Zhang, H.Q. et al. The effect of cold stress on endogenous hormones and CBF 1 homolog in four contrasting bamboo species. J For Res 17, 72–78 (2012). https://doi.org/10.1007/s10310-011-0253-x

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