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Effect of fluoride pollution on genetic variability of Larix gmelinii (Pinaceae) in East Siberia

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Journal of Forest Research

Abstract

The genetic variability of four natural populations of Larix gmelinii from East Siberia was determined and compared by RAPD analysis. Comparison of the RAPD profiles provided an estimation of variability in 193 RAPD fragments. More than 89% of these fragments were found to be polymorphic. The main genetic variability parameters of the two populations from Central Yakutia, a region free of fluoride pollution, had considerably higher values than those from East Transbaikalia, a region potentially affected by fluoride pollution (FLU, near a fluorite quarry growing on soils with a high natural content of fluorides). AMOVA revealed that 72.94% of the variation was within populations, while only 7.05% of the variation was between populations within geographical regions. The genetic diversity of the FLU fluoride-tolerant population was the lowest, but only slightly lower than that of a fluoride non-tolerant population from Chita, 50 km distant from FLU. Although this study demonstrates the absence of fundamental alterations of genetic structure within the populations of L. gmelinii growing on soils with a high content of fluorides, it is presumed that the reduction of genetic diversity was the genetic response of the FLU population to such an environmental stress as a constantly high concentration of fluorides within the soil.

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Acknowledgments

We thank two anonymous reviewers for providing comments that improved this manuscript. The financial support of the study was provided by the Integration Project of the Far East Branch of the Russian Academy of Sciences (RAS) No. 06-11–CO-06-023, and was partly supported by the Siberian Branch of RAS interdisciplinary grant No. 53, 5.18.

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Authors and Affiliations

  1. Institute of Biology and Soil Sciences, Far Eastern Branch of the Russian Academy of Sciences, Vladivostok, 690022, Russia

    Marina M. Kozyrenko & Elena V. Artyukova

  2. Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch of the Russian Academy of Sciences, Irkutsk, 664033, Russia

    Vladimir N. Shmakov & Yuri M. Konstantinov

Authors
  1. Marina M. Kozyrenko
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  2. Elena V. Artyukova
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  3. Vladimir N. Shmakov
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  4. Yuri M. Konstantinov
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Correspondence to Marina M. Kozyrenko.

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Kozyrenko, M.M., Artyukova, E.V., Shmakov, V.N. et al. Effect of fluoride pollution on genetic variability of Larix gmelinii (Pinaceae) in East Siberia. J For Res 12, 388–392 (2007). https://doi.org/10.1007/s10310-007-0031-y

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  • DOI: https://doi.org/10.1007/s10310-007-0031-y

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