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Journal of Forest Research

, Volume 11, Issue 5, pp 329–335 | Cite as

Micropropagation of red sanders (Pterocarpus santalinus L.) using mature nodal explants

  • Ekambaranellore Prakash
  • Patan Shaik Sha Valli KhanEmail author
  • Thoguru Jonh Vivek Sreenivasa Rao
  • Elu Singh Meru
ORIGINAL ARTICLE

Abstract

In vitro clonal multiplication of Pterocarpus santalinus L. was achieved using mature nodal explants of a 10-year-old elite quality tree. Combinations of serial transfer technique and incorporation of antioxidants (250 mg/l L-ascorbic acid and 50 mg/l citric acid) into the culture medium helped to minimize medium browning and improve explant survival during shoot sprouting. About 70% of explants were sprouted on Murashige and Skoog (MS) liquid medium containing 4.4 µM 6-benzyladenine (BA). The explant harvest period also influenced the bud break and shoot sprouting in nodal explants. The combination of 4.4 µM BA and 2.2 µM thidiazuron (TDZ) was found to be the most suitable growth regulator for obtaining the highest percentage of nodal segment sprouting (74%–75%), the number of secondary shoots per primary shoot (two or three), the shoot length (5–6 cm), the number of new nodal segments generated per active explant (four or five), and the multiplication coefficient (3.5) within 6 weeks. Repeated subculturing of nodal explants obtained from shoot cultures enabled continuous production of healthy axillary shoots. At the end of the sixth passage, about 90% of nodal explants produced five or six healthy green shoots, each being about 6.6 cm long with six or seven nodes. Multiplication coefficient was also increased from the first subculture (5.4) to the sixth subculture (8.3). The best rooting response was achieved on solidified half-strength MS medium supplemented with 4.9 µM indole-3-butyric acid (IBA). About 70% of the micropropagated plantlets were established successfully in 20-cm pots containing a mixture of soil and farmyard manure (4 : 1 ratio) and formed new leaflets.

Key words

Fabaceae Micropropagation Pterocarpus santalinus Red sanders Serial transfer technique 

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Copyright information

© The Japanese Forest Society and Springer-Verlag Tokyo 2006

Authors and Affiliations

  • Ekambaranellore Prakash
    • 1
    • 2
  • Patan Shaik Sha Valli Khan
    • 3
    • 4
    Email author
  • Thoguru Jonh Vivek Sreenivasa Rao
    • 1
    • 5
  • Elu Singh Meru
    • 1
  1. 1.Biotechnology Research CentreTirupatiIndia
  2. 2.Division of Biotechnology and Pharmaceuticals ResearchNational Health Research InstitutesTaiwanROC
  3. 3.Medicinal Plants and Horticultural Resources Division, Institute of Bioresources and Sustainable DevelopmentTakeylpat Institutional AreaImphalIndia
  4. 4.BIOFAR-EVACRP-Gabriel LippmannBelvauxLuxembourg
  5. 5.Department of AgronomyUniversity of KentuckyLexingtonUSA

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