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Analyse phytochimique et évaluation de l’activité antioxydante des échantillons du miel marocain

Phytochemical analysis and evaluation of the antioxidant activity of Moroccan honey samples

  • Biothérapie
  • Published:
Phytothérapie

Résumé

Dans le présent travail, quatre échantillons de miel collectés des régions différentes sont analysés pour leur teneur en composés phénoliques et testés pour leur activité antioxydante. Les contenus en polyphénols totaux ont été déterminés par la méthode de Folin-Ciocalteu et les teneurs en flavonoïdes par la méthode de trichlorure d’aluminium (AlCl3). L’activité antioxydante a été évaluée par la méthode de balayage du radical 2,2-diphényl-1-picrylhydrazyl. L’estimation quantitative des flavonoïdes et des phénols totaux par la méthode colorimétrique a montré que le miel est riche en ces composés avec une différence significative entre les échantillons. Les teneurs en polyphénols s’étendent entre 124,60 ± 1,12 et 75,14 ± 0,78 mg EAG/g de miel ; alors que les pourcentages des flavonoïdes sont compris entre 43,24 ± 0,72 et 19,64 ± 0,83 mg EQ/g de miel. L’évaluation quantitative de l’effet antioxydant a montré que l’échantillon de Zoumi est le plus actif (IC50 = 48,15 μg/ml), cette valeur ne présente pas une différence significative avec les deux témoins positifs (acide ascorbique : IC50 = 27,20 μg/ml et Trolox : IC50 = 43,72 μg/ml). Nous avons trouvé une corrélation positive très élevée (R2 = 0,974) entre les teneurs en flavonoïdes et la capacité antioxydante, alors que cette corrélation est moins élevée (R2 = 0,821) entre l’activité antioxydante et les phénols totaux.

Abstract

In the present work, four samples of honey collected from different regions are analyzed for phenol content and tested for their antioxidant activity. The total phenol content was determined by the Folin-Ciocalteu method and the flavonoids contents by the aluminium trichloride (AlCl3) method. The antioxidant activity was evaluated by the scanning method of radical diphenyl-1-picrylhydrazyl (DPPH). Quantitative estimation of total phenols and flavonoids content by a colorimetric method showed that honey is rich in these compounds with a significant difference between samples. The total phenol content ranges between 124.60 ± 1.12 and 75.14 ± 0.78 mg EAG/g of honey. At the same time, the percentages of flavonoids contents are between 43.24 ± 0.72 and 19.64 ± 0.83 mg EQ/g of honey. The quantitative evaluation of the antioxidant effect showed that the sample of Zoumi is most active (IC50 = 48.15 μg/ml), this value does not present a significant difference with the two positive controls (ascorbic acid: IC50 = 27.20 mg/ml and Trolox: IC50 = 43.72 μg/ml). We found a very high positive correlation (R2 = 0.974) between the flavonoids content and the antioxidant capacity, whereas this correlation was moderate (R2 = 0.821) between the antioxidant activity and the total phenol contents.

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Authors and Affiliations

  1. Laboratoire de biochimie-immunologie, département de biologie, faculté des sciences, université Mohammed-V, Rabat, Maroc

    A. Bouyahya, A. Et-Touys, F. Lagrouh, N. Dakka & Y. Bakri

  2. Laboratoire de biologie et santé, département de biologie, faculté des sciences, université Abdelmalek-Essaadi, Tétouan, Maroc

    A. Bouyahya & J. Abrini

Authors
  1. A. Bouyahya
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  2. J. Abrini
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  3. A. Et-Touys
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  4. F. Lagrouh
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  5. N. Dakka
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  6. Y. Bakri
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Correspondence to A. Bouyahya.

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Bouyahya, A., Abrini, J., Et-Touys, A. et al. Analyse phytochimique et évaluation de l’activité antioxydante des échantillons du miel marocain. Phytothérapie (2017). https://doi.org/10.1007/s10298-017-1122-3

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  • DOI: https://doi.org/10.1007/s10298-017-1122-3

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