Skip to main content
SpringerLink
Log in

Botanical survey, phytochemical investigation, and antibacterial activity of aqueous extract of Enantia polycarpa (DC) Engl. and Diels stem bark against methicillin resistant Staphylococcus aureus (MRSA)

Étude botanique, analyse phytochimique et évaluation de l’activité antibactérienne de l’extrait aqueux des écorces de Enantia polycarpa (DC) Engl. et Diels. vis-à-vis de Staphylococcus aureus résistant à la méticilline (SARM)

  • Pharmacognosie
  • Published:
Phytothérapie

Abstract

The present study was carried out to make botanical description, chemical analysis, and to evaluate the antibacterial activity of the aqueous extract from Enantia polycarpa (DC) Engl. and Diels stem bark against methicillin resistant Staphylococcus aureus (MRSA) strains isolated from clinical specimen. The methods of agar well diffusion and microbroth dilution in liquid media were used for susceptibility testing and minimum inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) determination. The tests were performed on eleven MRSA strains isolated from patients and one reference (ATCC 25923) strain. Enantia polycarpa (Annonaceae) is used in Africa to treat many diseases. The aqueous extract of this plant showed inhibitory activity against all tested bacteria and described zones of inhibition between 8.33 ± 0.58 mm and 16.33 ± 0.58 mm at 25 mg/ml. The lowest MIC and MBC values were observed with MRSA 564 UB/15(3.125 mg/ml and 12.5 mg/ml, respectively). The aqueous stem bark extract of Enantia polycarpa had a bactericidal activity on 75% of MRSA strains tested. The phytochemical analysis showed the presence of saponins, alkaloids, and tannins in aqueous extract. This could justify the antibacterial activity and the use of Enantia polycarpa stem bark in the treatment of various diseases in traditional society.

Resumé

La présente étude a été effectuée pour faire la description botanique, l’analyse phytochimique et évaluer l’activité antibactérienne de l’extrait aqueux des écorces de tige de Enantia polycarpa (DC) Engl. et Diels contre des souches cliniques de Staphylococcus aureus résistant à la méticilline (SARM) isolées de divers produits biologiques. Les méthodes de diffusion dans les puits et de microdilution en milieu liquide ont été utilisées pour le test de sensibilité et la détermination des CMI et CMB. Les tests ont été exécutés sur 11 souches de SARM isolées de patients et une souche de référence (ATCC 25923). Enantia polycarpa (Annonaceae) est utilisé en Afrique pour traiter plusieurs maladies. L’extrait aqueux de cette plante a montré une activité inhibitrice contre toutes les souches bactériennes testées et induit des zones d’inhibition comprises entre 8,33 ± 0,58 mm et 16,33 ± 0,58 mm à 25 mg/ml. Les valeurs des CMI et CMB les plus basses ont été observées avec SARM 564 UB/15 (3,125 et 12,5 mg/ml). Cet extrait a montré une activité bactéricide sur 75 % de souches de SARM testées. L’analyse phytochimique a montré la présence des saponines, des alcaloïdes et des tanins dans l’extrait aqueux. Cela pourrait justifier l’activité antibactérienne et l’utilisation des écorces de tige de Enantia polycarpa dans le traitement de diverses maladies en usage traditionnel.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Khosravi A, Behzadi A (2006) Evaluation of the antibacterial activity of the seed hull of Quercus barantii on some gramnegative bacteria. Pak J Med Sci 22:429–32

    Google Scholar 

  2. Kim HB, Jang HC, Nam HC, et al (2004) In vitro activities of 28 antibacterial agents against Staphylococcus aureus isolates from tertiary-care hospitals in Korea: a nationwide survey. Antimicrob Agents Chemother 48:1124–27

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Shittu AO, Lin J (2006) Antimicrobial susceptibility patterns and characterization of clinical isolates of Staphylococcus aureus in KwaZulu-Natal province, South Africa. BMC Inf Dis 6:125

    Article  Google Scholar 

  4. Perry J, Staley T (1997) Microbiology dynamics and diversity. Harcourt Brace College publishers, California, USA. 781 p

    Google Scholar 

  5. Fang H, Hedin G (2003) Rapid screening and identification of methicillin-resistant Staphylococcus aureus from clinical samples by selective-broth and real-time PCR assay. J Clin Microb 41:2894–99

    Article  CAS  Google Scholar 

  6. Durand G, Bes M, Meugnier H, et al (2006) Detection of new methicillin-resistant Staphylococcus aureus clones containing the toxic shock syndrome toxin 1 gene responsible for hospital and community-acquired infections in France. J Clin Microbiol 44:847–53

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Brown DFJ (2001) Detection of methicillin/oxacillin resistance in Staphylococci. J Antimicrob Chemother 48:65–70

    Article  CAS  PubMed  Google Scholar 

  8. Akoua K, Guessennd N, Gbonon V, et al (2004) Methicillin resistance of Staphylococcus in Abidjan 1998–2001: a new problem. Med Mal Infect 34:132–6

    Article  Google Scholar 

  9. Montesinos I, Saldo E, Delgado T, et al (2002) Epidemiology genotyping of methicillin-resistant Staphylococcus aureus by pulsed-field gel electrophoresis at a university hospital and comparison with antibiotyping and protein A and coagulase gene polymorphisms. J Clin Microbiol 40:2119–25

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Gould IM (2005) The clinical significance of methicillin-resistant Staphylococcus aureus. J Hosp Infect 61:277–82

    Article  CAS  PubMed  Google Scholar 

  11. Ambé ASA, Ouattara D, Tiébré MS, et al (2015) Diversity of medicinal plants used in the traditional treatment of the diarrhea on the markets of Abidjan (Côte d’Ivoire). JAPS 26:4081–96

    Google Scholar 

  12. Zirihi GN, Kra AM, Guédé-Guina F (2003) Evaluation of the antifungal activity of Microglossa pyrifolia (Lamarck) O. Kunze (Asteraceae) PYMI on the in vitro growth of Candida albicans. Rev Med Pharm Afr 17:11–8

    Google Scholar 

  13. CASFM (2014) Committee of the antibiogramme of the French Company of microbiology: recommendations 2014. French Company of Microbiology, Paris, France, 114 p

  14. Wiegand I, Hilpert K, Hancock REW (2007) Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances. Nat Protoc 3:163–75

    Article  Google Scholar 

  15. Nagababu P, Umamaheswara RV (2014) Phytochemical, antibacterial and antioxidant evaluation of Ceriops decandra (Griff). Ding Hou leaf extract. J Chem Pharm Res 6:428–37

    Google Scholar 

  16. Durgesh D, Wasnik P, Tumane M (2014) Preliminary phytochemical screening and evaluation of antibacterial activity of Portulaca oleracea L. against multiple drug resistant (MDR) pathogens isolated from clinical specimen. WJ Pharm Res 3:920–31

    Google Scholar 

  17. Shakil S, Mohd S, Rizvi D, et al (2015) Combating multi-drug resistance in Escherichia coli and Staphylococcus aureus with methanolic flower extracts of Spilanthes oleracae and estimating its phytochemical constitutes. W J Pharm Res 4:1867–87

    Google Scholar 

  18. Ponce AG, Fritz R, Del Alle C, et al (2003) Antimicrobial activity of essential oil on the native microflora of organic Swiss chard. Lebens-Wiss und Tech 36:679–84

    Article  CAS  Google Scholar 

  19. Riffel A, Medina LF, Stefani V, et al (2002) In vitro antimicrobial activity of a new series of 1,4-naphthoquinones. Braz J Med Biol Res 35:811–18

    Article  CAS  PubMed  Google Scholar 

  20. Suffredini IB, Bacchi EM, Sakuda TMK, et al (2002) Antibacterial activity of Apocynaceae extracts and MIC of Tabernaemontana angulata stem organic extract. Rev Bras Ciênc Farm 38:89–94

    Article  Google Scholar 

  21. N’Guessan JD, Dinzedi MR, Guessennd N, et al (2007) Antibacterial activity of the aqueous extract of Thonningia sanguinea against extended spectrum-β-lactamases (ESBL) producing Escherichia coli and Klebsiella pneumonia strains. Trop J Pharm Res 6:779–83

    Google Scholar 

  22. Faucherell Avril JL (2002) General and medical bacteriology. Edit Ellips 122p

    Google Scholar 

  23. Berche P, Gaillard JL, Simonet M (1991) Bactériologie: bactéries des infections humaines. Éditions Flammarion Médecine–Sciences, coll. « biol clin », Paris 1988, pp 230–8

    Google Scholar 

  24. Sofowora H (1993) Screening plants for bioactive agents: medicinal plants and traditional medicine in Africa. Spectrum Books Ltd., Sunshine House, Ibadan, Nigeria, 2nd edition, pp 134–56

    Google Scholar 

  25. Bowman WC, Rand MJ (1980) Textbook of pharmacology. Blackwell Scientific Publications, Oxford, 2nd edition, 5, 1928 p

    Google Scholar 

  26. Bouquet A, Debray M (1974) Medicinal plants of Côte d’Ivoire. Mémoires ORSTOM, 32, Paris, 232 p

    Google Scholar 

  27. Atindehou KK, Schmid C, Brun R, et al (2004) Antitrypanosomal and antiplasmodial activity of medicinal plants from Côted’Ivoire. J Ethnopharmacol 90:221–7

    Article  Google Scholar 

  28. Anosa GN, Udegbunam R, Okoro J (2014) In vivo antimalarial activities of Enantia polycarpa stem bark against Plasmodium berghei berghei in mice. J Ethnopharmacol 153:531–34

    Article  PubMed  Google Scholar 

  29. Buzas A, Egnell C, Orsay F (1965) On the presence of quinidine next to alkaloids berberinics in the barks of Enantia pilosa and Enantia polycarpa. Ann Pharm Fr 23:351–54

    CAS  PubMed  Google Scholar 

  30. Leboeuf M, Cave A (1972) Alkaloids of Annonaceae. Plant Med Phytother 6:87–90

    CAS  Google Scholar 

  31. Krishna PM, Rao KNV, Sandhya S, et al (2012) Review on phytochemical, ethnomedical and pharmacological studies on genus Sophora, Fabaceae. Rev Bras Farmacogn 22:1145–54

    Article  CAS  Google Scholar 

  32. Scabert A (1991) Antimicrobial properties of tannins. Phytochemistry 30:3875–83

    Article  Google Scholar 

  33. Elegami AA, El-Nima EI, El Tohami MS, et al (2002) Antimicrobial activity of some species of the family Combretaceae. Phytother Res 16:555–65

    Article  CAS  PubMed  Google Scholar 

  34. Séreme A, Millogo-Rasolodimby J, Guinko S, et al (2008) Therapeutics properties of the plants with tannins of Burkina Faso. Pharm Med Trad Afr 15:41–9

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratoire de botanique, UFR biosciences, université Félix-Houphouët-Boigny, 22, BP 582, Abidjan 22, Ivory Coast

    A. S. A. Ambé, D. Ouattara, Y. Kanga, K. Béné, G. N. Zirihi & K. E. N’Guessan

  2. Laboratoire de bactériologie–virologie, institut Pasteur de Côte-d’Ivoire, 08, BP 1563, Abidjan 08, Ivory Coast

    A. S. A. Ambé, N. K. Guessennd, F. K. Konan, Y. Kanga & K. Béné

Authors
  1. A. S. A. Ambé
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. N. K. Guessennd
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. D. Ouattara
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. F. K. Konan
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Y. Kanga
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. K. Béné
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. G. N. Zirihi
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. K. E. N’Guessan
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to K. E. N’Guessan.

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ambé, A.S.A., Guessennd, N.K., Ouattara, D. et al. Botanical survey, phytochemical investigation, and antibacterial activity of aqueous extract of Enantia polycarpa (DC) Engl. and Diels stem bark against methicillin resistant Staphylococcus aureus (MRSA). Phytothérapie 15, 267–273 (2017). https://doi.org/10.1007/s10298-016-1057-4

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10298-016-1057-4

Keywords

Mots clés

Search

Navigation