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Effet de l’huile fixe de la nigelle (Nigella sativa L.) sur le profil des androgènes chez le rat male

Effect of Nigella sativa fixed oil on the hormonal profile of androgens and circulating in male rats

  • Article Original
  • Pharmacologie
  • Published:
Phytothérapie

Résumé

Les extraits des graines de la nigelle sont largement utilisés dans la médecine traditionnelle. Nous avons étudié l’effet de l’huile fixe de la nigelle sur les taux plasmatiques de la testostérone (T), de l’hormone folliculostimulante (FSH), de l’hormone lutéinisante (LH) et sur le poids corporel et celui des organes reproducteurs chez le rat. Vingt rats mâles ont été divisés en deux groupes de dix: le groupe témoin et celui traité avec l’huile de la nigelle (0,5 ml/kg) par voie intrapéritonéale pendant 30 jours. Nos résultats ont montré que l’huile de la nigelle augmente significativement la masse des organes reproducteurs et les concentrations plasmatiques de la T, de la FSH et de la LH comparativement aux animaux témoins (p < 0,05). L’induction de la stéroïdogenèse observée pourrait être médiée par une activation de l’axe hypothalamohypophysogonadique.

Abstract

Nigella sativa L. seeds are used as herbal medicine for the treatment and prevention of several diseases. We investigated the effect of Nigella sativa fixed oil on the circulating levels of testosterone (T), follicle stimulating hormone (FSH) and lutenising hormone (LH), and on weight of body and reproductive organs in rats. Twenty male rats were divided into two groups: a vehicle-treated control group and an intraperitoneally treated group with Nigella sativa oil at a dose of 0.5 ml/kg body weight for 30 days. The Nigella sativa oil induced a significant increase in the weight of reproductive organs, and in the circulating levels of T, FSH and LH as compared to control animals (P < 0.05). We concluded that the Nigella sativa fixed oil may stimulate steroidogenesis by activation of hypothalamo-pituitary-testicular axis.

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Références

  1. Abidi P, Leers-Sucheta S, Azhar S (2004) Suppression of steroidogenesis and activator protein-1 transcription factor activity in rat adrenals by vitamin E deficiency induced chronic oxidative stress. J Nutr Biochem 15: 210–219

    Article  PubMed  CAS  Google Scholar 

  2. Agarwal S, Chauhan S, Mathur R (1986) Antifertility effects of embelin in male rats. Andrologia 18: 125–131

    Article  Google Scholar 

  3. Al-Saleh IA, Billedo G, El-Doush II (2006) Levels of selenium, DL-α-tocopherol, DL-γ-tocopherol, all-trans-retinol, thymoquinone and thymol in different brands of Nigella sativa seeds. J Food Comp Analys 19: 167–175

    Article  CAS  Google Scholar 

  4. Aybek H, Aybek Z, Rota S, et al. (2008) The effects of diabetes mellitus, age and vitamin E on testicular oxidative stress. Fertil Steril 90: 755–760

    Article  PubMed  CAS  Google Scholar 

  5. Barella L, Rota C, Stocklin E, Rimbach G (2006) α-tocopherol affects androgen metabolism in male rat. Ann N Y Acad Sci 1031: 334–336

    Article  Google Scholar 

  6. Brain RF, Homady MH, Mainardi M (1983) Preputial glands, dominance and aggressiveness in mice. Poll Zoo 50: 173–187

    Google Scholar 

  7. Burits M, Bucar F (2000) Antioxidant activity of Nigella sativa essential oil. Phytoth Res 14: 323–328

    Article  CAS  Google Scholar 

  8. Cemek M, Enginar H, Karaca T, Unak P (2006) In vivo radioprotective effects of Nigella sativa L. oil and reduced glutathione against irradiation-induced oxidative injury and number of peripheral blood lymphocytes in rats. Photochem Photobiol 82: 1691–1696

    PubMed  CAS  Google Scholar 

  9. Cheikh-Rouhou S, Besbes S, Lognay G, et al. (2008) Sterol composition of black cumin (Nigella sativa L.) and Aleppo pine (Pinus halepensis Mill.) seed oil. J Food Comp Analys 21: 162–168

    Article  CAS  Google Scholar 

  10. Choudhary A, Steinberger E (1975) Effect of 5α-reducted androgen on sex accessory organs, initiation and maintenance of spermatogenesis in the rat. Biol Reprod 12: 609–617

    Article  Google Scholar 

  11. Doroshkovich NA, Antsulevich SN, Vinogradov VV (1991) Effect of alpha-tocopherol on adrenal cortex functions under stress. UKR Biokhim Zh 63: 79–83

    Google Scholar 

  12. Eling FJ (1963) In: Mantagra W, Ellis RA, Silver AFO (eds) Advances in the Biology of Skin. Pergamon Press, Oxford, vol IV, pp. 2000–2219

    Google Scholar 

  13. Evans HM, Burr GO (1925) The anti-sterility vitamin fat soluble E. Proc Natl Acad Sci 11: 134–141

    Google Scholar 

  14. Ghannadi A, Haj Hashemi V, Jafarabadi J (2005) An investigation of the analgesic and anti-inflammatory effects of Nigella sativa seed polyphenols. J Med Food 8: 488–493

    Article  PubMed  CAS  Google Scholar 

  15. Ghedira K (2006) La nigelle cultivée: Nigella sativa L. (Ranunculaceae). Phytothérapie 4: 220–226

    Article  Google Scholar 

  16. Ghosh D, Das UB, Misro M (2002) Protective role of alpha-to-copherol-succinate (provitamin E) in cyclophosphamide induced testicular gametogenic and steroidogenic disorders: a correlative approach to oxidative stress. Free Radic Res 36: 1209–1218

    Article  PubMed  CAS  Google Scholar 

  17. Gilani AH, Jabeen K, Khan MAU (2004) A review of medicinal uses and pharmacological activities of Nigella sativa. Pakist J Biol Sc 7: 441–451

    Article  Google Scholar 

  18. Haq A, Lobo PI, Al-Tufail M, et al. (1999) Immunomodulatory effect of Nigella sativa proteins fractionated by ion exchange chromatography. Inter J Immun 21: 283–295

    Article  CAS  Google Scholar 

  19. Hartman TJ, Dorgan JF, Virtamo J, et al. (1999) Association between serum α-tocopherol and serum androgens and estrogens in older men. Nutr Cancer 35: 10–15

    Article  PubMed  CAS  Google Scholar 

  20. Houcher Z, Boudiaf K, Benboubetra M, Houcher B (2007) Effects of methanolic extract and commercial oil of Nigella sativa L. on blood glucose and antioxidant capacity in alloxan-induced diabetic rats. Pteridines 18: 8–18

    CAS  Google Scholar 

  21. Kaiser UB, Conn PM, Chin WW (1997) Studies on gonadotropin-releasing hormone (GnRH) action using GnRH receptor-expressing pituitary cell lines. Endocr Rev 18: 46

    Article  PubMed  CAS  Google Scholar 

  22. Khanna T, Zaidi FA, Dandiya PC (1993) CNS and analgesic studies on Nigella sativa. Fitoterapia 64: 407–410

    CAS  Google Scholar 

  23. Koechlin-Romonatxo C (2006) Oxygen oxidative stress and antioxidant supplementation or an other way for nutrition in respiratory diseases. Nutr Clin Metab 20: 165–177

    Google Scholar 

  24. Lin JK, Weng MS (2006) Flavonoids as nutraceuticals. In: Grotewold E (ed) The science of flavonoids. Springer, p 217

  25. Manna PR, Wang XJ, Stocco DM (2003) Involvement of multiple transcription factors in the regulation of steroidogenic acute regulatory protein gene expression. Steroids 68: 1125–1134

    Article  PubMed  CAS  Google Scholar 

  26. Mason KE (1993) Differences in testis injury and repair after vitamin A deficiency, vitamin E deficiency, and inanition. Amer J Anat 52: 153–239

    Article  Google Scholar 

  27. Mather JP, Saez JM, Dray F, Haour F (1983) Vitamin E prolongs survival and function of porcine Leydig cells in culture. Acta Endocrinol (Copenh) 102: 470–475

    CAS  Google Scholar 

  28. Meral I, Yener Z, Kahraman T, Mert N (2001) Effect of Nigella sativa on glucose concentration, lipid peroxidation, anti-oxidant defence system and liver damage in experimentally-induced diabetic rabbits. J Vet Med A Physiol Pathol Clin Med 48: 593–599

    Article  PubMed  CAS  Google Scholar 

  29. Merfort I, Wray V, Barakat H (1997) Flavonol triglycosides from seeds of Nigella sativa. Phytochemistry 46: 359–363

    Article  CAS  Google Scholar 

  30. Mukhallad AM, Mohamad MJM, Hatham D (2009) Effects of black seeds (Nigella sativa) on spermatogenesis and fertility of male Albinos rats. Res J Med Med Sci 4: 386–390

    Google Scholar 

  31. Nair SC, Salomi MJ, Panikkar B, Panikkar KR (1991) Modulatory effects of Crocus sativus and Nigella sativa extracts on cisplatin-induced toxicity in mice. J Ethnopharmacol 31: 75–83

    Article  PubMed  CAS  Google Scholar 

  32. Nergiz C, Otles S (2003) Some characteristics of Nigella (Nigella sativa L.) seed cultivated in Egypt and its lipid profile. Food Chem 83: 63–68

    Article  Google Scholar 

  33. Payne AH, Hales DB (2004) Overview of steroidogenic enzymes in the pathway from cholesterol to active steroid hormones. Endocr Rev 25: 947–970

    Article  PubMed  CAS  Google Scholar 

  34. Payne AH, Youngblood GL (1995) Regulation of expression of steroidogenic enzymes in Leydig cells. Biol Reprod 52: 217–225

    Article  PubMed  CAS  Google Scholar 

  35. Quinn Robert DVM (2005) Comparing rat’s to human’s age: how old is my rat in people years? Nutrition 21: 775–777

    Article  PubMed  CAS  Google Scholar 

  36. Rajkapoor B, Anandan A, Javakar B (2002) Anti-ulcer effect of Nigella sativa Linn. against gastric ulcers in rats. Curr Sci 82: 177–179

    Google Scholar 

  37. Sahunturk V, Guclu C, Baycu C (2007) Protective effects of vitamin E on ethane dimethane sulfonate-induced testicular toxicity in rats. Asian J Androl 9: 117–124

    Article  Google Scholar 

  38. Senthil KJ, Banudevi S, Sharmila M, et al. (2004) Effects of vitamin C and E on PCB (Aroclor 1254) induced oxidative stress, androgen binding protein and lactate in rat Sertoli Cells. Reprod Toxicol 19: 202–208

    Google Scholar 

  39. Sokol RZ (2009) Endocrinology of male infertility: evaluation and treatment. Semin Reprod Med 27: 149–158

    Article  PubMed  Google Scholar 

  40. Soleimani Mehranjani M, Noorafshan A, Momeni HR, et al. (2009) Stereological study of the effects of vitamin E on testis structure in rats treated with para-nonylphenol. Asian J Androl 11: 508–516

    Article  PubMed  Google Scholar 

  41. Tenekoon KH, Jeevathayaparan S, Kurukulasooriya AP, Karunanayake EH (1991) Possible hepatotoxicity of Nigella sativa seeds and Dregea volubilis leaves. J Ethnopharmacol 31: 283–289

    Article  Google Scholar 

  42. Vermar J, Nair A (2002) Effect of aflatoxins on testicular steroidogenesis and amelioration by vitamin E. Food Chem Toxicol 40: 669–672

    Article  Google Scholar 

  43. Wang X, Walsh LP, Reinhart AJ, Stocco DM (2000) The role of arachidonic acid in steroidogenesis and steroidogenic acute regulatory (StAR) gene and protein expression. J Biol Chem 275: 20204–20209

    Article  PubMed  CAS  Google Scholar 

  44. Zaoui A, Cherrah Y, Mahassini N, et al. (2002) Acute and chronic toxicity of Nigella sativa fixed oil. Phytomedicine 9: 69–74

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Équipe: hormones et métabolisme, laboratoire de physiopathologie et génétique moléculaire, faculté des sciences Ben-M’sik, université Hassan-II-Mohammedia, Casablanca, Maroc

    M. El Khasmi, A. Issaoub Allah, M. Farh, F. Riad, A. Safwate & E. H. Tahri

  2. Unité de radio-immuno-analyse, division des sciences du vivant, CNESTEN, BP 1382 RP, 10001, Rabat, Maroc

    N. El Abbadi

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  1. M. El Khasmi
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  2. A. Issaoub Allah
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  3. M. Farh
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  4. F. Riad
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  5. A. Safwate
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  6. N. El Abbadi
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  7. E. H. Tahri
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Correspondence to M. El Khasmi.

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El Khasmi, M., Issaoub Allah, A., Farh, M. et al. Effet de l’huile fixe de la nigelle (Nigella sativa L.) sur le profil des androgènes chez le rat male. Phytothérapie 9, 338–342 (2011). https://doi.org/10.1007/s10298-011-0654-1

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  • DOI: https://doi.org/10.1007/s10298-011-0654-1

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