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System-level understanding of gene expression and regulation for engineering secondary metabolite production in Streptomyces

  • Fermentation, Cell Culture and Bioengineering - Review
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

The gram-positive bacterium, Streptomyces, is noticed for its ability to produce a wide array of pharmaceutically active compounds through secondary metabolism. To discover novel bioactive secondary metabolites and increase the production, Streptomyces species have been extensively studied for the past decades. Among the cellular components, RNA molecules play important roles as the messengers for gene expression and diverse regulations taking place at the RNA level. Thus, the analysis of RNA-level regulation is critical to understanding the regulation of Streptomyces’ metabolism and secondary metabolite production. A dramatic advance in Streptomyces research was made recently, by exploiting high-throughput technology to systematically understand RNA levels. In this review, we describe the current status of the system-wide investigation of Streptomyces in terms of RNA, toward expansion of its genetic potential for secondary metabolite synthesis.

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Acknowledgements

This work was supported by Bio & Medical Technology Development Program (2018M3A9F3079664 to B.-K.C.) through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (MSIT). This work was also supported by a grant from the Novo Nordisk Foundation (NNF10CC1016517 to B.O.P).

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  1. Department of Biological Sciences and KI for the BioCentury, Korea Advanced Institute of Science and Technology, Daejeon, 34141, Republic of Korea

    Yongjae Lee, Namil Lee, Soonkyu Hwang, Kangsan Kim, Woori Kim, Jihun Kim, Suhyung Cho & Byung-Kwan Cho

  2. Department of Bioengineering, University of California San Diego, La Jolla, CA, 92093, USA

    Bernhard O. Palsson

  3. Department of Pediatrics, University of California San Diego, La Jolla, CA, 92093, USA

    Bernhard O. Palsson

  4. Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, 2800, Lyngby, Denmark

    Bernhard O. Palsson

  5. Intelligent Synthetic Biology Center, Daejeon, 34141, Republic of Korea

    Byung-Kwan Cho

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Lee, Y., Lee, N., Hwang, S. et al. System-level understanding of gene expression and regulation for engineering secondary metabolite production in Streptomyces. J Ind Microbiol Biotechnol 47, 739–752 (2020). https://doi.org/10.1007/s10295-020-02298-0

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