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Enhancing glutaric acid production in Escherichia coli by uptake of malonic acid

  • Metabolic Engineering and Synthetic Biology - Original Paper
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

Glutaric acid is an important organic acid applied widely in different fields. Most previous researches have focused on the production of glutaric acid in various strains using the 5-aminovaleric acid (AMV) or pentenoic acid synthesis pathways. We previously utilized a five-step reversed adipic acid degradation pathway (RADP) in Escherichia coli BL21 (DE3) to construct strain Bgl146. Herein, we found that malonyl-CoA was strictly limited in this strain, and increasing its abundance could improve glutaric acid production. We, therefore, constructed a malonic acid uptake pathway in E. coli using matB (malonic acid synthetase) and matC (malonic acid carrier protein) from Clover rhizobia. The titer of glutaric acid was improved by 2.1-fold and 1.45-fold, respectively, reaching 0.56 g/L and 4.35 g/L in shake flask and batch fermentation following addition of malonic acid. Finally, the highest titer of glutaric acid was 6.3 g/L in fed-batch fermentation at optimized fermentation conditions.

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Acknowledgements

This work was supported by the National Key R&D Program of China (2018YFA0901400), the National Natural Science Foundation of China (21877053, 31600044), the Open Project Program of China–Canada Joint Lab of Food Nutrition and Health, Beijing Technology and Business University (BTBU), and the Fundamental Research Funds for the Central Universities (JUSRP51705A, JUSRP11964).

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Authors and Affiliations

  1. National Engineering Laboratory for Cereal Fermentation Technology (NELCF), Jiangnan University, 1800 Lihu Road, Wuxi, 214122, Jiangsu, China

    Xue Sui, Mei Zhao, Guohui Li, Xiaojuan Zhang & Yu Deng

  2. China-Canada Joint Lab of Food Nutrition and Health (Beijing), Beijing Technology and Business University, Beijing, 100048, China

    Yingli Liu & Jing Wang

  3. Jiangsu Provincial Research Center for Bioactive Product Processing Technology, Jiangnan University, Wuxi 214122, Jiangsu, China

    Xue Sui, Mei Zhao, Guohui Li, Xiaojuan Zhang & Yu Deng

  4. The Open Project Program of China-Canada Joint Lab of Food Nutrition and Health, Beijing Technology and Business University (BTBU), Beijing 100048, China

    Yingli Liu & Jing Wang

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  1. Xue Sui
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  2. Mei Zhao
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  3. Yingli Liu
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  4. Jing Wang
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  5. Guohui Li
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  6. Xiaojuan Zhang
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  7. Yu Deng
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Correspondence to Guohui Li, Xiaojuan Zhang or Yu Deng.

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Sui, X., Zhao, M., Liu, Y. et al. Enhancing glutaric acid production in Escherichia coli by uptake of malonic acid. J Ind Microbiol Biotechnol 47, 311–318 (2020). https://doi.org/10.1007/s10295-020-02268-6

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  • DOI: https://doi.org/10.1007/s10295-020-02268-6

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