Bottom-up approaches in synthetic biology and biomaterials for tissue engineering applications

  • Mitchell S. Weisenberger
  • Tara L. DeansEmail author
Biotechnology Methods - Original Paper


Synthetic biologists use engineering principles to design and construct genetic circuits for programming cells with novel functions. A bottom-up approach is commonly used to design and construct genetic circuits by piecing together functional modules that are capable of reprogramming cells with novel behavior. While genetic circuits control cell operations through the tight regulation of gene expression, a diverse array of environmental factors within the extracellular space also has a significant impact on cell behavior. This extracellular space offers an addition route for synthetic biologists to apply their engineering principles to program cell-responsive modules within the extracellular space using biomaterials. In this review, we discuss how taking a bottom-up approach to build genetic circuits using DNA modules can be applied to biomaterials for controlling cell behavior from the extracellular milieu. We suggest that, by collectively controlling intrinsic and extrinsic signals in synthetic biology and biomaterials, tissue engineering outcomes can be improved.



We gratefully acknowledge the funding from the University of Utah startup funds, a University of Utah SEED Grant [10045314], the National Science Foundation CAREER program [CBET-1554017], the Office of Naval Research Young Investigator Program [N00014-16-1-3012], and the National Institutes of Health Trailblazer Award [1R21EB025413-01].


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Copyright information

© Society for Industrial Microbiology and Biotechnology 2018

Authors and Affiliations

  1. 1.Department of BioengineeringUniversity of UtahSalt Lake CityUSA

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