A novel riboregulator switch system of gene expression for enhanced microbial production of succinic acid

  • Jing Wang
  • Haoyuan Wang
  • Le Yang
  • Liping Lv
  • Zhe Zhang
  • Bin Ren
  • Lichun Dong
  • Ning Li
Metabolic Engineering and Synthetic Biology - Original Paper


In this paper, a novel riboregulator Switch System of Gene Expression including an OFF-TO-ON switch and an ON-TO-OFF switch was designed to regulate the expression state of target genes between “ON” and “OFF” by switching the identifiability of ribosome recognition site (RBS) based on the thermodynamic stability of different RNA–RNA hybridizations between RBS and small noncoding RNAs. The proposed riboregulator switch system was employed for the fermentative production of succinic acid using an engineered strain of E. coli JW1021, during which the expression of mgtC gene was controlled at “ON” state and that of pepc and ecaA genes were controlled at the “OFF” state in the lag phase and switched to the “OFF” and “ON” state once the strain enters the logarithmic phase. The results showed that using the strain of JW1021, the yield and productivity of succinic acid can reach 0.91 g g−1 and 3.25 g L−1 h−1, respectively, much higher than those using the strains without harboring the riboregulator switch system.


Switch of gene expression Gene regulation RBS Ribosome Succinic acid 



We thank the Dr. Rachel Chen (Georgia Tech, Atlanta, USA) for helping data analysis. This work is financially supported by the National Key R&D Program of China (2017YFB0603105), the National Natural Science Foundation of China (21606026, 21776025), the Fundamental Research Funds for the Central Universities (106112017CDJXY220005, 106112017CDJXF220009, 106112017CDJPT220001, 106112017CDJQJ228809, and 106112017CDJXFLX0014), and China Scholarship Council (201506050056).

Supplementary material

10295_2018_2019_MOESM1_ESM.docx (668 kb)
Supplementary material 1 (DOCX 667 kb)


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Copyright information

© Society for Industrial Microbiology and Biotechnology 2018

Authors and Affiliations

  1. 1.School of Chemistry and Chemical EngineeringChongqing UniversityChongqingPeople’s Republic of China
  2. 2.School of Chemistry and Chemical Engineering, Collaborative Innovation Center for Green Developmentin Wuling Moutain Area, Research Center for Environmental Monitoring, Hazard Prevention of Three Gorges ReservoirYangtze Normal UniversityChongqingPeople’s Republic of China
  3. 3.School of Chemical and Biomolecular Engineering and Renewable Bioproducts InstituteGeorgia Institute of TechnologyAtlantaUSA
  4. 4.Key Lahoratory of Catalysis and Functional Organic Molecule of Chongqing, College of Environment and ResourceChongqing Technology and Business UniversityChongqingPeople’s Republic of China
  5. 5.Key Laboratory of Low-grade Energy Utilization Technologies and Systems of the Ministry of EducationChongqing UniversityChongqingPeople’s Republic of China
  6. 6.Institute of Energy ResourcesHebei Academy of ScienceShijiazhuangPeople’s Republic of China

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