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A whole cell biocatalyst for double oxidation of cyclooctane

  • Biocatalysis - Short Communication
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

A novel whole cell cascade for double oxidation of cyclooctane to cyclooctanone was developed. The one-pot oxidation cascade requires only a minimum of reaction components: resting E. coli cells in aqueous buffered medium (=catalyst), the target substrate and oxygen as environmental friendly oxidant. Conversion of cyclooctane was catalysed with high efficiency (50% yield) and excellent selectivity (>94%) to cyclooctanone. The reported oxidation cascade represents a novel whole cell system for double oxidation of non-activated alkanes including an integrated cofactor regeneration. Notably, two alcohol dehydrogenases from Lactobacillus brevis and from Rhodococcus erythropolis with opposite cofactor selectivities and one monooxygenase P450 BM3 were produced in a coexpression system in one single host. The system represents the most efficient route with a TTN of up to 24363 being a promising process in terms of sustainability as well.

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Scheme 1
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Acknowledgements

We would like to thank Evonik Industries and the German Federal Ministry of Education and Research (BMBF; funding number 0316044A). We also thank Till Winkler and Prof. Dr. Werner Hummel (FZ Jülich) for providing the ADH enzymes, as well as Dr. Steffen Schaffer, Dr. Oliver Thum, Dr. Markus Pötter and Michaela Hauberg (Evonik Industries, Marl, Germany) for helpful discussions.

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Authors and Affiliations

  1. Institute of Biotechnology, RWTH Aachen University, Worringerweg 3, 52074, Aachen, Germany

    C. A. Müller, A. M. Weingartner, A. Dennig, A. J. Ruff & Ulrich Schwaneberg

  2. DWI–Leibniz Institut für Interaktive Materialien, Forckenbeckstraße 50, 52074, Aachen, Germany

    Ulrich Schwaneberg

  3. Faculty of Chemistry, Bielefeld University, Universitätsstrasse 25, 33615, Bielefeld, Germany

    H. Gröger

Authors
  1. C. A. Müller
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  2. A. M. Weingartner
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  3. A. Dennig
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  4. A. J. Ruff
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  5. H. Gröger
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  6. Ulrich Schwaneberg
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Correspondence to Ulrich Schwaneberg.

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C. A. Müller and A. M. Weingartner are shared authors.

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Müller, C.A., Weingartner, A.M., Dennig, A. et al. A whole cell biocatalyst for double oxidation of cyclooctane. J Ind Microbiol Biotechnol 43, 1641–1646 (2016). https://doi.org/10.1007/s10295-016-1844-5

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  • DOI: https://doi.org/10.1007/s10295-016-1844-5

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