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Enhancement of Penicillium echinulatum glycoside hydrolase enzyme complex

  • Bioenergy/Biofuels/Biochemicals
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

The enhancement of enzyme complex produced by Penicillium echinulatum grown in several culture media components (bagasse sugarcane pretreated by various methods, soybean meal, wheat bran, sucrose, and yeast extract) was studied to increment FPase, xylanase, pectinase, and β-glucosidase enzyme activities. The present results indicated that culture media composed with 10 g/L of the various bagasse pretreatment methods did not have any substantial influence with respect to the FPase, xylanase, and β-glucosidase attained maximum values of, respectively, 2.68 FPU/mL, 2.04, and 115.4 IU/mL. On the other hand, proposed culture media to enhance β-glucosidase production composed of 10 g/L steam-exploded bagasse supplemented with soybean flour 5.0 g/L, yeast extract 1.0 g/L, and sucrose 10.0 g/L attained, respectively, 3.19 FPU/mL and 3.06 IU/mL while xylanase was maintained at the same level. The proteomes obtained from the optimized culture media for enhanced FPase, xylanase, pectinase, and β-glucosidase production were analyzed using mass spectrometry and a panel of GH enzyme activities against 16 different substrates. Culture medium designed to enhance β-glucosidase activity achieved higher enzymatic activities values (13 measured activities), compared to the culture media for FPase/pectinase (9 measured activities) and xylanase (7 measured activities), when tested against the 16 substrates. Mass spectrometry analyses of secretome showed a consistent result and the greatest number of spectral counts of Cazy family enzymes was found in designed β-glucosidase culture medium, followed by FPase/pectinase and xylanase. Most of the Cazy identified protein was cellobiohydrolase (GH6 and GH7), endoglucanase (GH5), and endo-1,4-β-xylanase (GH10). Enzymatic hydrolysis of hydrothermally pretreated sugarcane bagasse performed with β-glucosidase enhanced cocktail achieved 51.4 % glucose yield with 10 % w/v insoluble solids at enzyme load of 15 FPU/g material. Collectively the results demonstrated that it was possible to rationally modulate the GH activity of the enzymatic complex secreted by P. echinulatum using adjustment of the culture medium composition. The proposed strategy may contribute to increase enzymatic hydrolysis of lignocellulosic materials.

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Acknowledgments

The authors thank Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for financial support. The authors gratefully acknowledge the provision of time at the CNPEM facility MAS at LNBio. The authors also thank Dr AJP Dillon for the strain P. echinulatum S01M29.

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Authors and Affiliations

  1. Brazilian Laboratory of Science and Technology of Bioethanol, Giuseppe Maximo Scolfaro 10.000, Campinas, SP, CEP 13083-970, Brazil

    Patrícia dos Santos Costa, Fernanda Büchli, Diogo Robl, Priscila da Silva Delabona, Sarita Candida Rabelo & José Geraldo da Cruz Pradella

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  1. Patrícia dos Santos Costa
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  2. Fernanda Büchli
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  3. Diogo Robl
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  4. Priscila da Silva Delabona
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  5. Sarita Candida Rabelo
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  6. José Geraldo da Cruz Pradella
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Correspondence to José Geraldo da Cruz Pradella.

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dos Santos Costa, P., Büchli, F., Robl, D. et al. Enhancement of Penicillium echinulatum glycoside hydrolase enzyme complex. J Ind Microbiol Biotechnol 43, 627–639 (2016). https://doi.org/10.1007/s10295-016-1746-6

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  • DOI: https://doi.org/10.1007/s10295-016-1746-6

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