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A novel chimeric prophage vB_LdeS-phiJB from commercial Lactobacillus delbrueckii subsp. bulgaricus

  • Genetics and Molecular Biology of Industrial Organisms
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

Prophage vB_LdeS-phiJB (phiJB) was induced by mitomycin C and UV radiation from the Lactobacillus delbrueckii subsp. bulgaricus SDMCC050201 isolated from a Chinese yoghurt sample. It has an isometric head and a non-contractile tail with 36,969 bp linear double-stranded DNA genome, which is classified into the group a of Lb. delbrueckii phages. The genome of phiJB is highly modular with functionally related genes clustered together. Unexpectedly, there is no similarity of its DNA replication module to any phages that have been reported, while it consists of open-reading frames homologous to the proteins of Lactobacillus strains. Comparative genomic analysis indicated that its late gene clusters, integration/lysogeny modules and DNA replication module derived from different evolutionary ancestors and integrated into a chimera. Our results revealed a novel chimeric phage of commercial Lb. delbrueckii and will broaden the knowledge of phage diversity in the dairy industry.

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Acknowledgments

We would like to thank M. van de Guchte for the kindness of providing Lb. delbrueckii subsp. bulgaricus ATCC11842. This work was supported by a grant of National Natural Science Foundation of China (no. 31271905, 31471715) and China Postdoctoral Science Foundation Funded Project (no. 2014M550364).

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  1. State Key Laboratory of Microbial Technology, Shandong University, 27 Shanda Nanlu, Jinan, 250100, People’s Republic of China

    Tingting Guo, Chenchen Zhang, Yongping Xin, Min Xin & Jian Kong

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  1. Tingting Guo
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  2. Chenchen Zhang
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Correspondence to Jian Kong.

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Guo, T., Zhang, C., Xin, Y. et al. A novel chimeric prophage vB_LdeS-phiJB from commercial Lactobacillus delbrueckii subsp. bulgaricus . J Ind Microbiol Biotechnol 43, 681–689 (2016). https://doi.org/10.1007/s10295-016-1739-5

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