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Secretome analysis of the thermophilic xylanase hyper-producer Thermomyces lanuginosus SSBP cultivated on corn cobs

  • Genetics and Molecular Biology of Industrial Organisms
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

Thermomyces lanuginosus is a thermophilic fungus known for its ability to produce industrially important enzymes including large amounts of xylanase, the key enzyme in hemicellulose hydrolysis. The secretome of T. lanuginosus SSBP was profiled by shotgun proteomics to elucidate important enzymes involved in hemicellulose saccharification and to characterise the presence of other industrially interesting enzymes. This study reproducibly identified a total of 74 proteins in the supernatant following growth on corn cobs. An analysis of proteins revealed nine glycoside hydrolase (GH) enzymes including xylanase GH11, β-xylosidase GH43, β-glucosidase GH3, α-galactosidase GH36 and trehalose hydrolase GH65. Two commercially produced Thermomyces enzymes, lipase and amylase, were also identified. In addition, other industrially relevant enzymes not currently explored in Thermomyces were identified including glutaminase, fructose-bisphosphate aldolase and cyanate hydratase. Overall, these data provide insight into the novel ability of a cellulase-free fungus to utilise lignocellulosic material, ultimately producing a number of enzymes important to various industrial processes.

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Acknowledgments

The work conducted at the Durban University of Technology (DUT) was supported by grants from DUT and the National Research Foundation, Republic of South Africa. The work conducted by the Joint BioEnergy Institute was supported by the Office of Science, Office of Biological and Environmental Research, of the US Department of Energy under Contract No. DE-AC02-05CH11231.

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Authors and Affiliations

  1. Department of Biotechnology and Food Technology, Durban University of Technology, Durban, 4001, South Africa

    A. M. Winger, K. Permaul & S. Singh

  2. Physical Biosciences Division, Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, USA

    J. L. Heazlewood, L. J. G. Chan & C. J. Petzold

Authors
  1. A. M. Winger
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  2. J. L. Heazlewood
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  3. L. J. G. Chan
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  4. C. J. Petzold
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  5. K. Permaul
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  6. S. Singh
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Correspondence to S. Singh.

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Winger, A.M., Heazlewood, J.L., Chan, L.J.G. et al. Secretome analysis of the thermophilic xylanase hyper-producer Thermomyces lanuginosus SSBP cultivated on corn cobs. J Ind Microbiol Biotechnol 41, 1687–1696 (2014). https://doi.org/10.1007/s10295-014-1509-1

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  • DOI: https://doi.org/10.1007/s10295-014-1509-1

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