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Purification and characterization of a novel alkaline β-1,3-1,4-glucanase (lichenase) from thermophilic fungus Malbranchea cinnamomea

  • Biocatalysis
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

A novel alkaline β-1,3-1,4-glucanase (McLic1) from a thermophilic fungus, Malbranchea cinnamomea, was purified and biochemically characterized. McLic1 was purified to homogeneity with a purification fold of 3.1 and a recovery yield of 3.7 %. The purified enzyme was most active at pH 10.0 and 55 °C, and exhibited a wide range of pH stability (pH 4.0–10.0). McLic1 displayed strict substrate specificity for barley β-glucan, oat β-glucan and lichenan, but did not show activity towards other tested polysaccharides and synthetic p-nitrophenyl derivates, suggesting that it is a specific β-1,3-1,4-glucanase. The K m values for barley β-glucan, oat β-glucan and lichenan were determined to be 0.69, 1.11 and 0.63 mg mL−1, respectively. Moreover, the enzyme was stable in various non ionic surfactants, oxidizing agents and several commercial detergents. Thus, the alkaline β-1,3-1,4-glucanase may have potential in industrial applications, such as detergent, paper and pulp industries.

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Acknowledgments

This study was financially supported by the National Science Fund for Distinguished Young Scholars (No. 31325021) and National Natural Science Foundation of China (No. 31071508).

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Authors and Affiliations

  1. Department of Biotechnology, College of Food Science and Nutritional Engineering, China Agricultural University, No.17 Qinghua Donglu, Haidian District, PO Box 294, Beijing, 100083, China

    Shaoqing Yang, Hao Xiong, Hongye Yang & Zhengqiang Jiang

  2. Bioresource Utilization Laboratory, College of Engineering, China Agricultural University, Beijing, 100083, China

    Qiaojuan Yan

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  1. Shaoqing Yang
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  2. Hao Xiong
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  3. Qiaojuan Yan
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  4. Hongye Yang
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Correspondence to Zhengqiang Jiang.

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Yang, S., Xiong, H., Yan, Q. et al. Purification and characterization of a novel alkaline β-1,3-1,4-glucanase (lichenase) from thermophilic fungus Malbranchea cinnamomea . J Ind Microbiol Biotechnol 41, 1487–1495 (2014). https://doi.org/10.1007/s10295-014-1494-4

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  • DOI: https://doi.org/10.1007/s10295-014-1494-4

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