Abstract
The real-time distribution of anticancer 1403C in fermentation broth of marine fungus Halorosellinia sp. was investigated. It was closely related with pH variations, which was, 1403C in the supernatant decreased while that in the mycelia increased with pH rising. There was only 0.5 % of the total 1403C left in the supernatant when pH reached 7.0. The scanning electron microscope then provided information that compounds precipitated on the mycelia when pH rose. Then, the pH-regulation experiments proved that 1403C mainly secreted extracellular and easily dissolved in acidic condition but precipitated and absorbed on the mycelia with the increase of broth pH. Thereby, a pH-regulation strategy was proposed and applied to accumulate 1403C on the mycelia before draw-off of fermentation broth. It significantly simplified purification process and is critical for 1403C preparation of industrial scale.
This is a preview of subscription content, log in via an institution to check access.
References
Bizukojc M, Pawlak M, Boruta T, Gonciarz J (2012) Effect of pH on biosynthesis of lovastatin and other secondary metabolites by Aspergillus terreus ATCC 20542. J Biotechnol 162:253–261
Bugni TS, Ireland CM (2004) Marine-derived fungi: a chemically and biologically diverse group. Nat Prod Rep 21:143–163
Damare S, Singh P, Raghukumar S (2010) Biotechnology of marine fungi. Prog Mol Subcell Biol 53:277–297
Debbab A, Aly AH, Proksch P (2011) Bioactive secondary metabolites from endophytes and associated marine-derived fungi. Fungal Divers 49:1–12
Fan L, Hou CL, Cannon PF, Li Y (2012) A new species of Melanospora on truffles from China. Mycologia 104:1433–1442
Jiang GC, Lin YC, Zhou SN, Vrijmoed LLP, Jones EBG (2000) Studies on the secondary metabolites of mangrove fungus No. 1403 from the South China Sea. Acta Sci Nat Univ Sun Yat-sen 39:68–72
Kang B, Zhang X, Wu Z, Qi H, Wang Z (2013) Effect of pH and nonionic surfactant on profile of intracellular and extracellular Monascus pigments. Process Biochem 48:759–767
Schmit JP, Shearer CA (2003) A checklist of mangrove-associated fungi, their geographical distribution and known host plants. Mycotaxon 85:423–477
Sridhar KR (2005) Diversity of fungi in mangrove ecosystems. Microbial diversity: current perspectives and potential applications. IK International Pvt. Ltd., New Delhi, pp 129–148
Teixeira JA, Correa TL, de Queiroz MV, de Araujo EF (2014) pH-dependent effect of pectinase secretion in Penicillium griseoroseum recombinant strains. J Basic Microbiol 54:133–141
Vera C, Guerrero C, Illanes A (2011) Determination of the transgalactosylation activity of Aspergillus oryzae β-galactosidase: effect of pH, temperature, and galactose and glucose concentrations. Carbohydr Res 346:745–752
Xie G, Zhu X, Li Q, Gu M, He Z, Wu J, Li J, Lin Y, Li M, She Z, Yuan J (2010) SZ-685C, a marine anthraquinone, is a potent inducer of apoptosis with anticancer activity by suppression of the Akt/FOXO pathway. Br J Pharmacol 159:689–697
Zhou W, Cai M, Zhou J, Jiang T, Wang M, Zhou X, Zhang Y (2013) Nutrition and bioprocess development for efficient biosynthesis of an antitumor compound from marine-derived fungus. J Ind Microbiol Biotechnol 40:1131–1142
Acknowledgments
This work was financially supported by the Chinese National High Technology Research and Development Program (2011AA090702, 2012AA092103, 2012AA092105). We thank Prof. Zhigang She, Sun Yat-Sen University for supplying the strain.
Author information
Authors and Affiliations
Corresponding author
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Zhou, W., Cai, M., Na, K. et al. pH-Dependent accumulation of anticancer compound on mycelia in fermentation of marine fungus. J Ind Microbiol Biotechnol 41, 1169–1173 (2014). https://doi.org/10.1007/s10295-014-1445-0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10295-014-1445-0