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The effect of albumin fusion structure on the production and bioactivity of the somatostatin-28 fusion protein in Pichia pastoris

  • Fermentation, Cell Culture and Bioengineering
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

Somatostatin, a natural inhibitor of growth hormone (GH), and its analogs have been used in clinical settings for the treatment of acromegaly, gigantism, thyrotropinoma, and other carcinoid syndromes. However, natural somatostatin is limited for clinical usage because of its short half-life in vivo. Albumin fusion technology was used to construct long-acting fusion proteins and Pichia pastoris was used as an expression system. Three fusion proteins (SS28)2-HSA, (SS28)3-HSA, and HSA-(SS28)2, were constructed with different fusion copies of somatostatin-28 and fusion orientations. The expression level of (SS28)3-HSA was much lower than (SS28)2-HSA and HSA-(SS28)2 due to the additional fusion of the somatostatin-28 molecule. MALDI-TOF mass spectrometry revealed that severe degradation occurred in the fermentation process. Similar to the standard, somatostatin-14, all three fusion proteins were able to inhibit GH secretion in blood, with (SS28)2-HSA being the most effective one. A pharmacokinetics study showed that (SS28)2-HSA had a prolonged half-life of 2 h. These results showed that increasing the number of small protein copies fused to HSA may not be a suitable method for improving protein bioactivity.

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Acknowledgments

The authors thank Dr. Jian Jin and his colleagues for their technical assistance in fermentation and purification processes. The work was financially supported by the Natural Science Foundation of Jiangsu Province (BK2012104 and BK2011165) and the Public Service Platform for Science and Technology Infrastructure Construction Project of Jiangsu Province (BM2012066).

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Authors and Affiliations

  1. Key Laboratory of Nuclear Medicine, Ministry of Health, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi, 214063, Jiangsu, China

    Yuedi Ding, Jun Fan, Wenxin Li, Ying Peng, Runlin Yang, Lili Deng & Qiang Fu

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  1. Yuedi Ding
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  2. Jun Fan
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  3. Wenxin Li
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  4. Ying Peng
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  6. Lili Deng
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  7. Qiang Fu
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Correspondence to Yuedi Ding.

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Ding, Y., Fan, J., Li, W. et al. The effect of albumin fusion structure on the production and bioactivity of the somatostatin-28 fusion protein in Pichia pastoris . J Ind Microbiol Biotechnol 41, 997–1006 (2014). https://doi.org/10.1007/s10295-014-1440-5

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  • DOI: https://doi.org/10.1007/s10295-014-1440-5

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