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Thermopressurized diluted phosphoric acid pretreatment of ligno(hemi)cellulose to make free sugars and nutraceutical oligosaccharides

  • Biotechnology Methods
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

Ligno(hemi)cellulosics (L(h)Cs) as sugarcane bagasse and loblolly pine sawdust are currently being used to produce biofuels such as bioethanol and biobutanol through fermentation of free sugars that are often obtained enzymatically. However, this bioconversion requires a pretreatment to solubilize the hemicellulose fractions, thus facilitating the action of the cellulolytic enzymes. Instead of the main free monosaccharides used in these current models, the modulation of thermopressurized orthophosphoric acid as a pretreatment, in the ranges of 3–12 atm and pH 1.5–2.5, can produce nondigestible oligosaccharides (NDOS) such as xylo-oligosaccharides (XOS) because heteroxylan is present in both types of hardwood and softwood hemicelluloses. A comparative thin-layer chromatographic analysis of the hydrolytic products showed the best conditions for NDOS production to be 7 atm/water, pH 2.25 and 2.50, and 8.5 atm/water for both sources. Particular hydrolysates from 7 atm (171 °C) at pHs 2.25 and 2.50 both for cane bagasse and pine sawdust, with respective oligosaccharide contents of 57 and 59 %, once mixed in a proportion of 1:1 for each plant source, were used in vitro as carbon sources for Bifidobacterium or Lactobacillus. Once both bacteria attained the stationary phase of growth, an unforeseen feature emerged: the preference of B. animalis for bagasse hydrolysates and, conversely, the preference of L. casei for pine hydrolysates. Considering the fact that nutraceutical oligosaccharides from both hemicelluloses correspond to higher value-added byproducts, the technology using a much diluted thermopressurized orthophosphoric acid pretreatment becomes an attractive choice for L(h)Cs.

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Acknowledgments

The authors would like to thank the National Council for Scientific and Technological Development (CNPq), the Coordination for the Improvement of Higher Education Personnel (CAPES), and the Araucaria Foundation from SETI-PR.

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Authors and Affiliations

  1. Department of Pharmacy, Federal University of Paraná (UFPR), Curitiba, PR, 80210-170, Brazil

    Marcela Tiboni & José Domingos Fontana

  2. Department of Chemistry and Biology, Federal Technological University of Paraná (UTFPR), Curitiba, PR, 81280-340, Brazil

    Adelia Grzybowski, Gizele Rejane Baldo, Edson Flausino Dias Jr. & José Domingos Fontana

  3. Department of Chemical Engineering, California Institute of Technology (Caltech), 210-41, Pasadena, CA, 91125, USA

    Robert D. Tanner & Julia Ann Kornfield

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  1. Marcela Tiboni
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  2. Adelia Grzybowski
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  3. Gizele Rejane Baldo
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  4. Edson Flausino Dias Jr.
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  7. José Domingos Fontana
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Correspondence to José Domingos Fontana.

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Tiboni, M., Grzybowski, A., Baldo, G.R. et al. Thermopressurized diluted phosphoric acid pretreatment of ligno(hemi)cellulose to make free sugars and nutraceutical oligosaccharides. J Ind Microbiol Biotechnol 41, 957–964 (2014). https://doi.org/10.1007/s10295-014-1426-3

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  • DOI: https://doi.org/10.1007/s10295-014-1426-3

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