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Anaerobic ferrous oxidation by heterotrophic denitrifying enriched culture

  • Environmental Microbiology
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

Heterotrophic denitrifying enriched culture (DEC) from a lab-scale high-rate denitrifying reactor was discovered to perform nitrate-dependent anaerobic ferrous oxidation (NAFO). The DEC was systematically investigated to reveal their denitrification activity, their NAFO activity, and the predominant microbial population. The DEC was capable of heterotrophic denitrification with methanol as the electron donor, and autotrophic denitrification with ferrous salt as the electron donor named NAFO. The conversion ratios of ferrous-Fe and nitrate-N were 87.41 and 98.74 %, and the consumption Fe/N ratio was 2.3:1 (mol/mol). The maximum reaction velocity and half saturation constant of Fe were 412.54 mg/(l h) and 8,276.44 mg/l, and the counterparts of N were 20.87 mg/(l h) and 322.58 mg/l, respectively. The predominant bacteria were Hyphomicrobium, Thauera, and Flavobacterium, and the predominant archaea were Methanomethylovorans, Methanohalophilus, and Methanolobus. The discovery of NAFO by heterotrophic DEC is significant for the development of wastewater treatment and the biogeochemical iron cycle and nitrogen cycle.

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Acknowledgments

Financial support for this work by the National Natural Science Foundation of China (51278457) and Zhejiang Provincial National Science Foundation (Z5110094) are greatly appreciated.

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Authors and Affiliations

  1. Department of Environmental Engineering, Zhejiang University, Hangzhou, 310058, People’s Republic of China

    Ru Wang, Ping Zheng, Ya-Juan Xing, Meng Zhang, Abbas Ghulam, Wei Li & Lan Wang

  2. Quzhou College, Quzhou, 324000, People’s Republic of China

    Zhi-qing Zhao

  3. Department of Chemical Engineering, University of Gujrat, Gujrat, Pakistan

    Abbas Ghulam

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  1. Ru Wang
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  2. Ping Zheng
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  3. Ya-Juan Xing
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Correspondence to Ping Zheng.

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Wang, R., Zheng, P., Xing, YJ. et al. Anaerobic ferrous oxidation by heterotrophic denitrifying enriched culture. J Ind Microbiol Biotechnol 41, 803–809 (2014). https://doi.org/10.1007/s10295-014-1424-5

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  • DOI: https://doi.org/10.1007/s10295-014-1424-5

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