Analysis of the bovine rumen microbiome reveals a diversity of Sus-like polysaccharide utilization loci from the bacterial phylum Bacteroidetes
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Several unique Sus-like polysaccharide utilization loci (PULs) were identified from bacteria resident in bovine rumen microbiomes through functional screening of a fosmid library. The loci were phylogenetically assigned to the genus Prevotella within the phylum Bacteroidetes. These findings were augmented by a bioinformatic re-evaluation of ruminal Prevotella genomes, revealing additional loci not previously reported in the literature. Analysis of Bacteroidales-affiliated genomes reconstructed from a bovine rumen metagenome in a previous study further expanded the diversity of Sus-like PULs resident in this microbiome. Our findings suggest that Sus-like systems represent an important mechanism for degradation of a range of plant-derived glycans in ruminants.
KeywordsBacteroidetes Carbohydrate Active Enzyme Fosmid Library Functional Screening Fosmid Clone
This research was supported by funds provided to Meat and Livestock Australia project number B.CCH.1005, as part of the Reducing Emissions from Livestock Research Program, and by a Marie Curie International Incoming Fellowship from the European Commission (awarded to PBP; PIIF-GA-2010-274303). We are grateful to Honglei Chen for preparation of amplicons for 454 pyrosequencing, to Ivan Gregor and Alice McHardy for assistance with PhyloPythiaS, and to Nigel Tomkins for assistance with sample collection.
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