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High-yield production of the VP1 structural protein epitope from serotype O foot-and-mouth disease virus in Escherichia coli

  • Fermentation, Cell Culture and Bioengineering
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

For effective control of foot-and-mouth disease (FMD), the development of rapid diagnostic systems and vaccines are required against its etiological agent, FMD virus (FMDV). To accomplish this, efficient large-scale expression of the FMDV VP1 protein, with high solubility, needs to be optimized. We attempted to produce high levels of a serotype O FMDV VP1 epitope in Escherichia coli. We identified the subtype-independent serotype O FMDV VP1 epitope sequence and used it to construct a glutathione S-transferase (GST) fusion protein. For efficient production of the FMDV VP1 epitope fused to GST (VP1e–GST), four E. coli strains and three temperatures were examined. The conditions yielding the greatest level of VP1e–GST with highest solubility were achieved with E. coli BL21(DE3) at 25 °C. For high-level production, fed-batch cultures were conducted in 5-l bioreactors. When cells were induced at a high density and complex feeding solutions were supplied, approximately 11 g of VP1e–GST was obtained from a 2.9-l culture. Following purification, the VP1 epitope was used to immunize rabbits, and we confirmed that it induced an immune response.

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Acknowledgments

This research was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MEST) (No. 20120005476).

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Authors and Affiliations

  1. Department of Chemical and Biomolecular Engineering, KAIST, Daejeon, Republic of Korea

    Joon-Goo Jung, Yong Jae Lee, Natarajan Velmurugan & Ki Jun Jeong

  2. Institute for the BioCentury, KAIST, Daejeon, Republic of Korea

    Ki Jun Jeong

  3. Animal, Plant and Fisheries Quarantine and Inspection Agency, Gyeonggi-do, Anyang city, Republic of Korea

    Young-Joon Ko & Hyang-Sim Lee

Authors
  1. Joon-Goo Jung
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  2. Yong Jae Lee
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  3. Natarajan Velmurugan
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  6. Ki Jun Jeong
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Correspondence to Ki Jun Jeong.

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Jung, JG., Lee, Y.J., Velmurugan, N. et al. High-yield production of the VP1 structural protein epitope from serotype O foot-and-mouth disease virus in Escherichia coli . J Ind Microbiol Biotechnol 40, 705–713 (2013). https://doi.org/10.1007/s10295-013-1273-7

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