Cloning of a novel feruloyl esterase gene from rumen microbial metagenome and enzyme characterization in synergism with endoxylanases
- 1.1k Downloads
A feruloyl esterase (FAE) gene was isolated from a rumen microbial metagenome, cloned into E. coli, and expressed in active form. The enzyme (RuFae2) was identified as a type C feruloyl esterase. The RuFae2 alone released ferulic acid from rice bran, wheat bran, wheat-insoluble arabinoxylan, corn fiber, switchgrass, and corn bran in the order of decreasing activity. Using a saturating amount of RuFae2 for 100 mg substrate, a maximum of 18.7 and 80.0 μg FA was released from 100 mg corn fiber and wheat-insoluble arabinoxylan, respectively. Addition of GH10 endoxylanase (EX) synergistically increased the release of FA with the highest level of 6.7-fold for wheat bran. The synergistic effect of adding GH11 EX was significantly smaller with all the substrates tested. The difference in the effect of the two EXs was further analyzed by comparing the rate in the release of FA with increasing EX concentration using wheat-insoluble arabinoxylan as the substrate.
KeywordsFeruloyl esterase Ferulic acid esterase Ferulic acid Metagenomics Corn fiber Arabinoxylan
Reference to a company and/or products is only for purposes of information and does not imply approval of recommendation of the product to the exclusion of others that may also be suitable. All programs and services of the U.S. Department of Agriculture are offered on a nondiscriminatory basis without regard to race, color, national origin, religion, sex, age, marital status, or handicap.
- 6.Benoit I, Danchin EGJ, Blelchrodt R-J, de Vries RP (2008) Biotechnological applications and potential of fungal feruloyl esterases based on prevalence, classification and biochemical diversity. Biochemi Lett 30:387–396Google Scholar
- 25.Pell G, Taylor EJ, Gloster TM, Turkenburg JP, Fontes CM, Ferreira LM, Nagy T, Clark SJ, Davies GJ, Gilbert HJ (2004) The mechanism by which family 10 glycoside hydrolases bind decorated substrates. J Biol Chem 279:393–9597Google Scholar
- 28.Vandermarliere E, Bourgois TM, Rombouts S, Van Campenhout S, Wolckaert G, Strelkov SV, Delcour JA, Rabijns A, Courtin CM (2008) Crystallographic analysis shows substrate binding at the −3 to +1 active-site subsites and at the surface of glycoside hydrolase family 11 endo-1,4-beta xylanases. Biochem J 410:71–79PubMedCrossRefGoogle Scholar
- 31.Wong DWS (2010) Application of metagenomics for industrial bioproducts. In: Marco D (ed) Metagenomics theory methods and applications. Caister Academic Press, Norfolk, UK, pp 141–158Google Scholar